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Autophagy promotes programmed cell death and corpse clearance in specific cell types of the Arabidopsis root cap

View ORCID ProfileQiangnan Feng, View ORCID ProfileRiet De Rycke, View ORCID ProfileYasin Dagdas, View ORCID ProfileMoritz K. Nowack
doi: https://doi.org/10.1101/2022.02.16.480680
Qiangnan Feng
aDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium
bVIB-UGENT Center for Plant Systems Biology, 9052 Ghent, Belgium
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Riet De Rycke
aDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium
bVIB-UGENT Center for Plant Systems Biology, 9052 Ghent, Belgium
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Yasin Dagdas
cGregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna BioCenter (VBC), Vienna, Austria
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Moritz K. Nowack
aDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium
bVIB-UGENT Center for Plant Systems Biology, 9052 Ghent, Belgium
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  • For correspondence: moritz.nowack@vib.be
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Summary

Autophagy is a conserved quality control pathway that mediates the degradation of unnecessary or dysfunctional cellular components by targeting them to the lysosomes or central vacuoles. Autophagy has been implicated in the regulation or execution of regulated cell death processes in a wide range of eukaryotes. However, its function in developmentally controlled programmed cell death (dPCD) in plants remains little studied and controversial. Here, we investigated the role of autophagy in dPCD using the Arabidopsis root cap as an accessible and genetically tractable model system. We show that autophagic flux is induced prior to dPCD execution in both root cap tissues, the columella and the lateral root cap (LRC), and impaired in autophagy-deficient mutants. These mutants show a strongly delayed cell death and an absence of corpse clearance in the columella during and after their shedding into the rhizosphere. However, autophagy deficiency does not affect dPCD execution or corpse clearance in LRC cells at the distal end of the root cap. Our results demonstrate that autophagy promotes dPCD in a highly cell-type specific manner, and present the root cap as a powerful model system to study organ-specific autophagy in vivo.

Competing Interest Statement

The authors have declared no competing interest.

  • Abbreviations

    PCD
    programmed cell death
    dPCD
    developmental PCD
    LRC
    lateral root cap
    RCD
    regulated cell death
    ConcA
    Concanamycin A
    FDA
    fluorescein diacetate
    PI
    propidium iodide
    TE
    tracheary element
    ToIM
    tonoplast integrity marker.
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    Posted February 18, 2022.
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    Autophagy promotes programmed cell death and corpse clearance in specific cell types of the Arabidopsis root cap
    Qiangnan Feng, Riet De Rycke, Yasin Dagdas, Moritz K. Nowack
    bioRxiv 2022.02.16.480680; doi: https://doi.org/10.1101/2022.02.16.480680
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    Autophagy promotes programmed cell death and corpse clearance in specific cell types of the Arabidopsis root cap
    Qiangnan Feng, Riet De Rycke, Yasin Dagdas, Moritz K. Nowack
    bioRxiv 2022.02.16.480680; doi: https://doi.org/10.1101/2022.02.16.480680

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