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Comparing lipid remodeling in mouse adipose and liver tissue with quantitative Raman microscopy

View ORCID ProfileAlexandra Paul, View ORCID ProfileBelén Chanclón, View ORCID ProfileCecilia Brännmark, View ORCID ProfilePernilla Wittung-Stafshede, View ORCID ProfileCharlotta S. Olofsson, View ORCID ProfileIngrid Wernstedt Asterholm, View ORCID ProfileSapun H. Parekh
doi: https://doi.org/10.1101/2022.03.07.483297
Alexandra Paul
1Department of Biology and Biological Engineering, Division of Chemical Biology, Chalmers University of Technology, Kemivägen 10, 412 96 Gothenburg, Sweden
2Department of Biomedical Engineering, University of Texas at Austin, 107 W Dean Keeton Street, Austin, TX 78712, USA
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Belén Chanclón
3Department of Physiology (Metabolic Physiology), Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, Box 432, 405 30 Gothenburg, Sweden
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Cecilia Brännmark
3Department of Physiology (Metabolic Physiology), Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, Box 432, 405 30 Gothenburg, Sweden
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Pernilla Wittung-Stafshede
1Department of Biology and Biological Engineering, Division of Chemical Biology, Chalmers University of Technology, Kemivägen 10, 412 96 Gothenburg, Sweden
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Charlotta S. Olofsson
3Department of Physiology (Metabolic Physiology), Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, Box 432, 405 30 Gothenburg, Sweden
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Ingrid Wernstedt Asterholm
3Department of Physiology (Metabolic Physiology), Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, Box 432, 405 30 Gothenburg, Sweden
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Sapun H. Parekh
2Department of Biomedical Engineering, University of Texas at Austin, 107 W Dean Keeton Street, Austin, TX 78712, USA
4Department of Molecular Spectroscopy, Max Planck Institute for Polymer Research, Ackermannweg 10, 55 128 Mainz, Germany
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  • For correspondence: sparekh@utexas.edu
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ABSTRACT

Brown adipose tissue (BAT) consists of highly metabolically active adipocytes that catabolize nutrients to produce heat. Playing an active role in triacylglycerol (TAG) clearance, research has shown that dietary fatty acids can modulate the TAG chemistry deposition in BAT after weeks-long dietary intervention, similar to what has been shown in white adipose tissue (WAT). Our objective was to compare the influence of sustained, non-chronic dietary intervention (a one-week interval) on WAT and BAT lipid metabolism and deposition in situ. We use quantitative, label-free chemical microscopy to show that one week of HFD intervention results in dramatically larger lipid droplet (LD) growth in BAT (and liver) compared to LD growth in inguinal WAT (IWAT). Moreover, BAT showed lipid remodeling as increased unsaturated TAGs in LDs, resembling the dietary lipid composition, while WAT (and liver) did not show lipid remodeling on this time scale. Concurrently, expression of genes involved in lipid metabolism, particularly desaturases, was reduced in BAT and liver from HFD-fed mice after one week. Our data show that BAT lipid chemistry remodels exceptionally fast to dietary lipid intervention compared WAT, which further points towards a role in TAG clearance.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted March 07, 2022.
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Comparing lipid remodeling in mouse adipose and liver tissue with quantitative Raman microscopy
Alexandra Paul, Belén Chanclón, Cecilia Brännmark, Pernilla Wittung-Stafshede, Charlotta S. Olofsson, Ingrid Wernstedt Asterholm, Sapun H. Parekh
bioRxiv 2022.03.07.483297; doi: https://doi.org/10.1101/2022.03.07.483297
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Comparing lipid remodeling in mouse adipose and liver tissue with quantitative Raman microscopy
Alexandra Paul, Belén Chanclón, Cecilia Brännmark, Pernilla Wittung-Stafshede, Charlotta S. Olofsson, Ingrid Wernstedt Asterholm, Sapun H. Parekh
bioRxiv 2022.03.07.483297; doi: https://doi.org/10.1101/2022.03.07.483297

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