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A happy accident: a novel turfgrass reference genome

View ORCID ProfileAlyssa R. Phillips, View ORCID ProfileArun S. Seetharam, Taylor AuBuchon-Elder, Edward S. Buckler, Lynn J. Gillespie, View ORCID ProfileMatthew B. Hufford, Victor Llaca, M. Cinta Romay, Robert J. Soreng, Elizabeth A. Kellogg, Jeffrey Ross-Ibarra
doi: https://doi.org/10.1101/2022.03.08.483531
Alyssa R. Phillips
1Dept. of Evolution and Ecology and Center for Population Biology, University of California, Davis, CA, USA, E-mail: ;
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  • For correspondence: rossibarra@ucdavis.edu arphillips@ucdavis.edu rossibarra@ucdavis.edu
Arun S. Seetharam
2Department of Ecology, Evolution, and Organismal Biology, Iowa State University, IA, USA
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Taylor AuBuchon-Elder
3Donald Danforth Plant Science Center, Olivette, MO, USA
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Edward S. Buckler
4School of Integrative Plant Sciences, Section of Plant Breeding and Genetics, Cornell University, Ithaca, NY, USA
5Institute for Genomic Diversity, Cornell University, Ithaca, NY, USA
6Agricultural Research Service, United States Department of Agriculture, Ithaca, NY, USA
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Lynn J. Gillespie
7Botany Section, Research and Collections, Canadian Museum of Nature, Ottawa, ON, Canada
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Matthew B. Hufford
2Department of Ecology, Evolution, and Organismal Biology, Iowa State University, IA, USA
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Victor Llaca
8Corteva Agriscience, Johnston, IA, USA
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M. Cinta Romay
5Institute for Genomic Diversity, Cornell University, Ithaca, NY, USA
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Robert J. Soreng
9Deptartment of Botany, Smithsonian Institution, Washington, DC, USA
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Elizabeth A. Kellogg
3Donald Danforth Plant Science Center, Olivette, MO, USA
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Jeffrey Ross-Ibarra
1Dept. of Evolution and Ecology and Center for Population Biology, University of California, Davis, CA, USA, E-mail: ;
10Genome Center, University of California, Davis, CA, USA
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  • For correspondence: rossibarra@ucdavis.edu arphillips@ucdavis.edu rossibarra@ucdavis.edu
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ABSTRACT

Poa pratensis, commonly known as Kentucky bluegrass, is a popular cool-season grass species used as turf in lawns and recreation areas globally. Despite its substantial economic value, a reference genome had not previously been assembled due to the genome’s relatively large size and biological complexity that includes apomixis, polyploidy, and interspecific hybridization. We report here a fortuitous de novo assembly and annotation of a P. pratensis genome. The draft assembly consists of 6.09 Gbp with an N50 scaffold length of 65.1 Mbp, and a total of 118 scaffolds, generated using PacBio long reads and Bionano optical map technology. We annotated 256K gene models and found 58% of the genome to be composed of transposable elements. To demonstrate the applicability of the reference genome, we evaluated population structure and estimated genetic diversity in three North American wild P. pratensis populations. Our results support previous studies that found high genetic diversity and population structure within the species. The reference genome and annotation will be an important resource for turfgrass breeding and biologists interested in this complex species.

Competing Interest Statement

The authors have declared no competing interest.

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  • ↵* Co-first authors

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted March 09, 2022.
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A happy accident: a novel turfgrass reference genome
Alyssa R. Phillips, Arun S. Seetharam, Taylor AuBuchon-Elder, Edward S. Buckler, Lynn J. Gillespie, Matthew B. Hufford, Victor Llaca, M. Cinta Romay, Robert J. Soreng, Elizabeth A. Kellogg, Jeffrey Ross-Ibarra
bioRxiv 2022.03.08.483531; doi: https://doi.org/10.1101/2022.03.08.483531
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A happy accident: a novel turfgrass reference genome
Alyssa R. Phillips, Arun S. Seetharam, Taylor AuBuchon-Elder, Edward S. Buckler, Lynn J. Gillespie, Matthew B. Hufford, Victor Llaca, M. Cinta Romay, Robert J. Soreng, Elizabeth A. Kellogg, Jeffrey Ross-Ibarra
bioRxiv 2022.03.08.483531; doi: https://doi.org/10.1101/2022.03.08.483531

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