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MUC5B mobilizes and MUC5AC spatially aligns mucociliary transport on human airway epithelium

Daniel Song, Ethan Iverson, Logan Kaler, Margaret A. Scull, View ORCID ProfileGregg A. Duncan
doi: https://doi.org/10.1101/2022.03.11.484020
Daniel Song
1Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742
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Ethan Iverson
2Department of Cell Biology & Molecular Genetics, University of Maryland, College Park, MD 20742
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Logan Kaler
3Biophysics Program, University of Maryland, College Park, MD 20742
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Margaret A. Scull
2Department of Cell Biology & Molecular Genetics, University of Maryland, College Park, MD 20742
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  • For correspondence: gaduncan@umd.edu scull@umd.edu
Gregg A. Duncan
1Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742
3Biophysics Program, University of Maryland, College Park, MD 20742
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  • ORCID record for Gregg A. Duncan
  • For correspondence: gaduncan@umd.edu scull@umd.edu
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Abstract

Airway mucus acts as a protective barrier and vehicle for clearance of pathogens, providing the lungs with a defense mechanism called mucociliary clearance (MCC). Airway mucus is composed of two mucins, mucin 5B (MUC5B) and 5AC (MUC5AC) that form a hydrogel that enables functional clearance in health. However, abnormalities in mucin expression, specifically increases in MUC5AC is observed in chronic respiratory diseases and leading to defective MCC. Our current understanding of MCC impairment focuses on mucin concentration, while the impact of mucin composition remains unclear. Here, we use MUC5AC/B-knock out (KO) human airway epithelial (HAE) tissue cultures to understand the role and contribution of individual secreted mucins on MCC mechanisms. We find that KO cultures result in impaired or discoordinated mucociliary transport demonstrating the importance of each of these mucins to effective MCC and shedding light on a new mechanism of mucin composition-dependent airway clearance.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted March 13, 2022.
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MUC5B mobilizes and MUC5AC spatially aligns mucociliary transport on human airway epithelium
Daniel Song, Ethan Iverson, Logan Kaler, Margaret A. Scull, Gregg A. Duncan
bioRxiv 2022.03.11.484020; doi: https://doi.org/10.1101/2022.03.11.484020
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MUC5B mobilizes and MUC5AC spatially aligns mucociliary transport on human airway epithelium
Daniel Song, Ethan Iverson, Logan Kaler, Margaret A. Scull, Gregg A. Duncan
bioRxiv 2022.03.11.484020; doi: https://doi.org/10.1101/2022.03.11.484020

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