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High salinity activates CEF and attenuates state transitions in both psychrophilic and mesophilic Chlamydomonas species

View ORCID ProfileIsha Kalra, Xin Wang, Ru Zhang, View ORCID ProfileRachael Morgan-Kiss
doi: https://doi.org/10.1101/2022.03.14.484132
Isha Kalra
aDepartment of Microbiology, Miami University, Oxford, Ohio 45056
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  • For correspondence: ishakalr@usc.edu
Xin Wang
aDepartment of Microbiology, Miami University, Oxford, Ohio 45056
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Ru Zhang
bDonald Danforth Plant Science Center, St. Louis, Missouri 63132
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Rachael Morgan-Kiss
aDepartment of Microbiology, Miami University, Oxford, Ohio 45056
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ABSTRACT

In the last decade, studies have revealed the importance of PSI-driven cyclic electron flow (CEF) in stress acclimation in model organisms like C. reinhardtii; however, these studies focused on transient, short-term stress. In addition, PSI-supercomplexes are associated with CEF during state transition response to short-term stress. On the other hand, the role of CEF during long-term stress acclimation is still largely unknown. In this study, we elucidate the involvement of CEF in acclimation response to long-term high salinity in three different Chlamydomonas species displaying varying salinity tolerance. We compared CEF rates, capacity for state transitions, and formation of supercomplexes after salinity acclimation in the model mesophile C. reinhardtii and two psychrophilic green algae C. priscuii (UWO241) and C. sp. ICE-MDV. CEF was activated under high salt in all three species, with the psychrophilic Chlamydomonas spp. exhibiting the highest CEF rates. High salt acclimation was also correlated with reduced state transition capacity and a PSI-supercomplex was associated with high CEF. We propose that under long-term stress, CEF is constitutively activated through assembly of a stable PSI-supercomplex. The proteomic composition of the long-term PSI-supercomplex is distinct from the supercomplex formed during state transitions, and its presence attenuates the state transition response.

Competing Interest Statement

The authors have declared no competing interest.

  • Abbreviations

    CEF
    cyclic electron flow
    PETC
    photosynthetic electron transport chain
    PSI
    photosystem I
    PSII
    photosystem II
    LHCI
    light harvesting complex I
    LHCII
    light harvesting complex 2
    Cyt b6f
    cytochrome b6f
    NPQ
    non-photochemical quenching
    FNR
    ferredoxin NADP reductase
    ANR1
    anaerobic response I protein
    CAS
    calcium sensing protein
    PGRL1
    proton gradient like protein 1
    ECS
    electrochomic shift
    DIRK
    dark interval relaxation kinetics
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    Posted March 16, 2022.
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    High salinity activates CEF and attenuates state transitions in both psychrophilic and mesophilic Chlamydomonas species
    Isha Kalra, Xin Wang, Ru Zhang, Rachael Morgan-Kiss
    bioRxiv 2022.03.14.484132; doi: https://doi.org/10.1101/2022.03.14.484132
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    High salinity activates CEF and attenuates state transitions in both psychrophilic and mesophilic Chlamydomonas species
    Isha Kalra, Xin Wang, Ru Zhang, Rachael Morgan-Kiss
    bioRxiv 2022.03.14.484132; doi: https://doi.org/10.1101/2022.03.14.484132

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