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Engineering Principles and Algorithmic Design Synthesis for Ultracompact Bio-Hybrid Perfusion Chip

View ORCID ProfileAmelie Erben, View ORCID ProfileThomas Kellerer, Josefine Lissner, Constanze Eulenkamp, View ORCID ProfileThomas Hellerer, View ORCID ProfileHauke-Clausen-Schaumann, View ORCID ProfileStefanie Sudhop, View ORCID ProfileMichael Heymann
doi: https://doi.org/10.1101/2022.03.16.484492
Amelie Erben
1Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, Lothstr. 34, 80533 Munich, Germany
2Heinz-Nixdorf-Chair of Biomedical Electronics, TranslaTUM, Campus Klinikum rechts der Isar Technical University of Munich, Einsteinstraße 25, 81675 Munich, Germany
3Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl Platz 1, 80539 Munich, Germany
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Thomas Kellerer
4Multiphoton Imaging Lab, Munich University of Applied Sciences, 80335 Munich, Germany
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Josefine Lissner
5Hyperganic Group GmbH, Georgenstraße 38, 80799 Munich, Germany
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Constanze Eulenkamp
6Department of Applied Sciences and Mechatronics, Munich University of Applied Sciences, Lothstr. 34, 80335 Munich, Germany
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Thomas Hellerer
4Multiphoton Imaging Lab, Munich University of Applied Sciences, 80335 Munich, Germany
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Hauke-Clausen-Schaumann
1Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, Lothstr. 34, 80533 Munich, Germany
3Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl Platz 1, 80539 Munich, Germany
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Stefanie Sudhop
1Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, Lothstr. 34, 80533 Munich, Germany
3Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl Platz 1, 80539 Munich, Germany
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Michael Heymann
7Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Pfaffenwaldring 57, 70569 Stuttgart, Germany
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  • For correspondence: michael.heymann@bio.uni-stuttgart.de
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Abstract

Bioinspired 3D microfluidic systems that combine vascularization with extracellular matrix architectures of organotypic geometry, composition and biophysical traits can help advance our understanding of microorgan physiology. Here, two-photon stereolithography is adopted to fabricate freestanding perfusable 3D cell scaffolds with micrometer resolution from gelatin methacryloyl hydrogel derived from extracellular matrix protein. As a proof of concept, we introduce an ultracompact bio-hybrid chip layout to demonstrate perfusion and cell seeding of double-digit μm proteinaceous channels. This perfusion chip consists of a standardized microfluidic interface fabricated from standard resin and a GM10 bioink channel printed atop of this interface. In addition, we demonstrate that algorithmic design synthesis can recapitulate intact alveoli and capillary networks with tunable design parameters to implement vascularized alveolar tissue models. This approach will allow for a systematic investigation of cell-cell and tissue dynamics in response to defined structural, mechanical and bio-molecular cues and is ultimately scalable to fabricate organ-on-a-chip systems.

Competing Interest Statement

Josefine Lissner is employee of Hyperganic Group GmbH

Footnotes

  • https://doi.org/10.18419/darus-2612

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted March 18, 2022.
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Engineering Principles and Algorithmic Design Synthesis for Ultracompact Bio-Hybrid Perfusion Chip
Amelie Erben, Thomas Kellerer, Josefine Lissner, Constanze Eulenkamp, Thomas Hellerer, Hauke-Clausen-Schaumann, Stefanie Sudhop, Michael Heymann
bioRxiv 2022.03.16.484492; doi: https://doi.org/10.1101/2022.03.16.484492
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Engineering Principles and Algorithmic Design Synthesis for Ultracompact Bio-Hybrid Perfusion Chip
Amelie Erben, Thomas Kellerer, Josefine Lissner, Constanze Eulenkamp, Thomas Hellerer, Hauke-Clausen-Schaumann, Stefanie Sudhop, Michael Heymann
bioRxiv 2022.03.16.484492; doi: https://doi.org/10.1101/2022.03.16.484492

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