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Topographic axonal projection at single-cell precision supports local retinotopy in the mouse superior colliculus

Dmitry Molotkov, Leiron Ferrarese, Tom Boissonnet, View ORCID ProfileHiroki Asari
doi: https://doi.org/10.1101/2022.03.25.485790
Dmitry Molotkov
1Epigenetics and Neurobiology Unit, EMBL Rome, European Molecular Biology Laboratory, Monterotondo, 00015, Italy
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Leiron Ferrarese
1Epigenetics and Neurobiology Unit, EMBL Rome, European Molecular Biology Laboratory, Monterotondo, 00015, Italy
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Tom Boissonnet
1Epigenetics and Neurobiology Unit, EMBL Rome, European Molecular Biology Laboratory, Monterotondo, 00015, Italy
2Collaboration for joint PhD degree between EMBL and Université Grenoble Alpes, Grenoble Institut des Neurosciences, La Tronche, 38700, France
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Hiroki Asari
1Epigenetics and Neurobiology Unit, EMBL Rome, European Molecular Biology Laboratory, Monterotondo, 00015, Italy
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  • ORCID record for Hiroki Asari
  • For correspondence: asari@embl.it
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Abstract

Retinotopy, like all long-range projections, can arise from the axons themselves or their targets, but the underlying connectivity pattern remains unknown at the fine scale. To address this question, we functionally mapped the spatial organization of the input axons and target neurons in the mouse retinocollicular pathway at single-cell resolution using in vivo two-photon calcium imaging. We found a near-perfect retinotopic tiling of retinal ganglion cell axon terminals, with an average error below 30 μm or 2 degrees of visual angle. The precision of retinotopy was relatively lower for local neurons in the superior colliculus. Subsequent data-driven modelling ascribed it to a low input convergence, on average 5.5 retinal ganglion cell inputs to a postsynaptic cell in the superior colliculus. These results indicate that retinotopy arises largely from topographically precise input from presynaptic cells, rather than elaborating local circuitry to reconstruct the topography by postsynaptic cells.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Competing interests: The authors declare no competing financial interests.

  • Full revision of the manuscript by adding more data on RGC axonal imaging, new data on SC recordings, as well as modelling results.

  • Acronyms

    2D
    two-dimensional
    AAV
    adeno-associated virus
    CNMF
    constrained non-negative matrix factorization
    RF
    receptive field
    RGC
    retinal ganglion cells
    SC
    superior colliculus
    SD
    standard deviation.
  • Copyright 
    The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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    Posted April 26, 2023.
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    Topographic axonal projection at single-cell precision supports local retinotopy in the mouse superior colliculus
    Dmitry Molotkov, Leiron Ferrarese, Tom Boissonnet, Hiroki Asari
    bioRxiv 2022.03.25.485790; doi: https://doi.org/10.1101/2022.03.25.485790
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    Topographic axonal projection at single-cell precision supports local retinotopy in the mouse superior colliculus
    Dmitry Molotkov, Leiron Ferrarese, Tom Boissonnet, Hiroki Asari
    bioRxiv 2022.03.25.485790; doi: https://doi.org/10.1101/2022.03.25.485790

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