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Zebrafish cornea formation and homeostasis reflect robustness in camera-type eye biology, regardless of its environment

Kaisa Ikkala, Sini Raatikainen, Frederic Michon
doi: https://doi.org/10.1101/2022.03.28.486026
Kaisa Ikkala
1Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland
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Sini Raatikainen
1Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland
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Frederic Michon
1Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland
3Institute for Neurosciences of Montpellier, Univ Montpellier, INSERM, Montpellier, France
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  • For correspondence: Frederic.michon@inserm.fr
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Abstract

The cornea is the transparent tissue covering the camera-type eyes. This position makes this tissue prone to environmental aggressions, and physical alteration can have a drastic negative impact on sight. The current knowledge on zebrafish corneal physiology is too scarce to get an understanding on its strategy to maintain a clear sight in aquatic environment. In this study, we focus on corneal formation and maturation in zebrafish. After describing the morphological changes taking place from 3 days post fertilization to adulthood, we analyzed cell proliferation. We show that label retaining cells appear just before 1 month of age. Our cell proliferation study, combined to the study of Pax6a and krtt1c19e expression, demonstrate a long maturation process. This process ends with a solid patterning of corneal innervation. Finally, corneal abrasion wound healing process recapitulates the maturation process, via a plasticity period. Altogether, our study decipher the maturation steps of an aquatic cornea. These findings will facilitate the use of zebrafish as model of corneal physiology studies.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted March 29, 2022.
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Zebrafish cornea formation and homeostasis reflect robustness in camera-type eye biology, regardless of its environment
Kaisa Ikkala, Sini Raatikainen, Frederic Michon
bioRxiv 2022.03.28.486026; doi: https://doi.org/10.1101/2022.03.28.486026
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Zebrafish cornea formation and homeostasis reflect robustness in camera-type eye biology, regardless of its environment
Kaisa Ikkala, Sini Raatikainen, Frederic Michon
bioRxiv 2022.03.28.486026; doi: https://doi.org/10.1101/2022.03.28.486026

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