Abstract
Limited chemical shift dispersion represents a major barrier to studying multi-state equilibria of large membrane proteins by 19F NMR. We describe a novel monofluoroethyl 19F probe with a dramatically larger chemical shift dispersion than existing probes. The improved conformational sensitivity and line shape enabled the detection of hidden states of a critical membrane transporter, guiding subsequent structural elucidation by single-particle cryo-electron microscopy.
Competing Interest Statement
The authors have declared no competing interest.
Copyright
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