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Evolutionary gain and loss of a plant pattern-recognition receptor for HAMP recognition

View ORCID ProfileSimon Snoeck, Bradley W. Abramson, Anthony G. K. Garcia, View ORCID ProfileAshley N. Egan, View ORCID ProfileTodd P. Michael, View ORCID ProfileAdam D. Steinbrenner
doi: https://doi.org/10.1101/2022.03.30.484633
Simon Snoeck
1Department of Biology, University of Washington, Seattle, Washington 98195, USA
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Bradley W. Abramson
2The Plant Molecular and Cellular Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
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Anthony G. K. Garcia
1Department of Biology, University of Washington, Seattle, Washington 98195, USA
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Ashley N. Egan
3Department of Biology, Utah Valley University, Orem, Utah 84058, USA
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Todd P. Michael
2The Plant Molecular and Cellular Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
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Adam D. Steinbrenner
1Department of Biology, University of Washington, Seattle, Washington 98195, USA
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  • For correspondence: astein10@uw.edu
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Abstract

Pattern recognition receptors (PRR) recognize distinct pathogen and herbivore-associated molecular patterns (PAMPs and HAMPs) and mediate activation of immune responses, but the evolution of new PRR sensing functions is not well understood. We employed comparative genomics and functional analysis to define evolutionary events leading to the sensing of the peptide HAMP inceptin (In11) by the PRR Inceptin Receptor (INR). Existing and de novo genome assemblies revealed that the presence of a functional INR gene corresponded with In11 response across 55 million years (my) of legume evolution, and that In11 recognition is unique to the clade of Phaseoloid legumes. The INR loci of certain Phaseoloid and non-Phaseoloid species also contain diverse INR-like homologues, suggesting that the evolution of INR receptor function ∼28 mya occurred after an ancestral gene insertion ∼32 mya. Functional analysis of chimeric and ancestrally reconstructed receptors revealed that specific AA differences in the C1 leucine-rich repeat (LRR) domain and C2 intervening motif likely mediated gain of In11 recognition. In summary, we present a conceptual model for the evolution of a defined PRR function based on patterns of INR variation in legumes.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted April 01, 2022.
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Evolutionary gain and loss of a plant pattern-recognition receptor for HAMP recognition
Simon Snoeck, Bradley W. Abramson, Anthony G. K. Garcia, Ashley N. Egan, Todd P. Michael, Adam D. Steinbrenner
bioRxiv 2022.03.30.484633; doi: https://doi.org/10.1101/2022.03.30.484633
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Evolutionary gain and loss of a plant pattern-recognition receptor for HAMP recognition
Simon Snoeck, Bradley W. Abramson, Anthony G. K. Garcia, Ashley N. Egan, Todd P. Michael, Adam D. Steinbrenner
bioRxiv 2022.03.30.484633; doi: https://doi.org/10.1101/2022.03.30.484633

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