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Cytoneme-mediated transport of active Wnt5b/Ror2 complexes in zebrafish gastrulation

Chengting Zhang, Lucy Brunt, Sally Rogers, View ORCID ProfileSteffen Scholpp
doi: https://doi.org/10.1101/2022.04.07.487468
Chengting Zhang
Living Systems Institute, School of Biosciences, College of Life and Environmental Sciences, University of Exeter
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Lucy Brunt
Living Systems Institute, School of Biosciences, College of Life and Environmental Sciences, University of Exeter
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Sally Rogers
Living Systems Institute, School of Biosciences, College of Life and Environmental Sciences, University of Exeter
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  • For correspondence: s.rogers@exeter.ac.uk s.scholpp@exeter.ac.uk
Steffen Scholpp
Living Systems Institute, School of Biosciences, College of Life and Environmental Sciences, University of Exeter
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  • ORCID record for Steffen Scholpp
  • For correspondence: s.rogers@exeter.ac.uk s.scholpp@exeter.ac.uk
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Summary

Chemical signaling is the primary means by which cells communicate in the embryo. The underlying principle refers to a group of ligand-producing cells and a group of cells that respond to this signal because they express the appropriate receptors. In the zebrafish embryo, Wnt5b binds to the receptor Ror2 to trigger the Wnt/Planar Cell Polarity (Wnt/PCP) signaling pathway to regulate tissue polarity and cell migration. However, it is still unclear how this lipophilic ligand is transported from the source cells through the aqueous extracellular space to the target tissue. Here we show that Wnt5b, together with Ror2, is loaded on long protrusions. The active Wnt5b/Ror2 complexes are handed over from these cytonemes to the receiving cell to trigger Wnt/PCP signaling, regardless of whether the cell expresses functional receptors. On the tissue level, we show that cytoneme-dependent spreading of active Wnt5b/Ror2 affects convergence and extension in the zebrafish gastrula.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted April 08, 2022.
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Cytoneme-mediated transport of active Wnt5b/Ror2 complexes in zebrafish gastrulation
Chengting Zhang, Lucy Brunt, Sally Rogers, Steffen Scholpp
bioRxiv 2022.04.07.487468; doi: https://doi.org/10.1101/2022.04.07.487468
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Cytoneme-mediated transport of active Wnt5b/Ror2 complexes in zebrafish gastrulation
Chengting Zhang, Lucy Brunt, Sally Rogers, Steffen Scholpp
bioRxiv 2022.04.07.487468; doi: https://doi.org/10.1101/2022.04.07.487468

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