Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Liver-secreted fluorescent blood plasma markers enable chronic imaging of microcirculation

Xiaowen Wang, Christine Delle, Antonis Asiminas, View ORCID ProfileSonam Akther, Marta Vittani, Peter Brøgger, Peter Kusk, Camilla Trang Vo, View ORCID ProfileAyumu Konno, View ORCID ProfileHirokazu Hirai, Masahiro Fukuda, View ORCID ProfilePia Weikop, View ORCID ProfileSteven A Goldman, View ORCID ProfileMaiken Nedergaard, View ORCID ProfileHajime Hirase
doi: https://doi.org/10.1101/2022.04.08.487498
Xiaowen Wang
1Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christine Delle
1Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Antonis Asiminas
1Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sonam Akther
1Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sonam Akther
Marta Vittani
1Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Peter Brøgger
1Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Peter Kusk
1Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Camilla Trang Vo
1Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ayumu Konno
2Viral Vector Core, Gunma University Initiative for Advanced Research, Maebashi, Gunma 371-8511, Japan
3Department of Neurophysiology & Neural Repair, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ayumu Konno
Hirokazu Hirai
2Viral Vector Core, Gunma University Initiative for Advanced Research, Maebashi, Gunma 371-8511, Japan
3Department of Neurophysiology & Neural Repair, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Hirokazu Hirai
Masahiro Fukuda
4Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore, 169857 Singapore
5International Research Center for Medical Sciences (IRCMS), Kumamoto University, Kumamoto, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pia Weikop
1Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Pia Weikop
Steven A Goldman
1Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
6Center for Translational Neuromedicine, University of Rochester Medical Center, Rochester, NY, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Steven A Goldman
Maiken Nedergaard
1Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
6Center for Translational Neuromedicine, University of Rochester Medical Center, Rochester, NY, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Maiken Nedergaard
  • For correspondence: maiken_nedergaard@urmc.rochester.edu hirase@sund.ku.dk
Hajime Hirase
1Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
6Center for Translational Neuromedicine, University of Rochester Medical Center, Rochester, NY, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Hajime Hirase
  • For correspondence: maiken_nedergaard@urmc.rochester.edu hirase@sund.ku.dk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Studying blood microcirculation is vital for gaining insights into vascular diseases. Acute administration of fluorescent tracers is currently used for deep tissue blood flow imaging. This is invasive, and the plasma fluorescence decreases within an hour of administration. We report a novel approach for the longitudinal study of vasculature. Using a single systemic administration of viral vectors, we express fluorescent secretory albumin-fusion proteins in the liver to label the blood in mice. All segments of the vasculature in brain and peripheral tissue are observable by two-photon microscopy within two weeks of vector administration. This approach allows for observation of circulation without the need for repeated administration for several months. We demonstrate the chronic assessment of vascular functions at micro-and mesoscopic scales. This genetic plasma labeling approach represents a versatile and cost-effective method for the chronic investigation of vasculature functions across the body in health and disease.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Sources of Funding: This work was supported by the Novo Nordisk Foundation (HaH, NNF19OC0058058), Danmarks Frie Forskningsfond (0134-00107B), the Olav Thon Foundation (MN), the Lundbeck Foundation (MN), the program for Brain Mapping by Integrated Neurotechnologies for Disease Studies (HiH, Brain/MINDS, JP20dm0207057 and JP21dm0207111) by the Japan Agency for Medical Research and development (AMED) and JSPS KAKENHI (AK,) by MEXT, Japan.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
Back to top
PreviousNext
Posted April 10, 2022.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Liver-secreted fluorescent blood plasma markers enable chronic imaging of microcirculation
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Liver-secreted fluorescent blood plasma markers enable chronic imaging of microcirculation
Xiaowen Wang, Christine Delle, Antonis Asiminas, Sonam Akther, Marta Vittani, Peter Brøgger, Peter Kusk, Camilla Trang Vo, Ayumu Konno, Hirokazu Hirai, Masahiro Fukuda, Pia Weikop, Steven A Goldman, Maiken Nedergaard, Hajime Hirase
bioRxiv 2022.04.08.487498; doi: https://doi.org/10.1101/2022.04.08.487498
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Liver-secreted fluorescent blood plasma markers enable chronic imaging of microcirculation
Xiaowen Wang, Christine Delle, Antonis Asiminas, Sonam Akther, Marta Vittani, Peter Brøgger, Peter Kusk, Camilla Trang Vo, Ayumu Konno, Hirokazu Hirai, Masahiro Fukuda, Pia Weikop, Steven A Goldman, Maiken Nedergaard, Hajime Hirase
bioRxiv 2022.04.08.487498; doi: https://doi.org/10.1101/2022.04.08.487498

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Physiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4383)
  • Biochemistry (9602)
  • Bioengineering (7097)
  • Bioinformatics (24869)
  • Biophysics (12624)
  • Cancer Biology (9962)
  • Cell Biology (14361)
  • Clinical Trials (138)
  • Developmental Biology (7961)
  • Ecology (12111)
  • Epidemiology (2067)
  • Evolutionary Biology (15990)
  • Genetics (10929)
  • Genomics (14745)
  • Immunology (9871)
  • Microbiology (23681)
  • Molecular Biology (9486)
  • Neuroscience (50889)
  • Paleontology (369)
  • Pathology (1540)
  • Pharmacology and Toxicology (2683)
  • Physiology (4019)
  • Plant Biology (8657)
  • Scientific Communication and Education (1510)
  • Synthetic Biology (2397)
  • Systems Biology (6440)
  • Zoology (1346)