Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

The GUN mutants: new weapons to unravel ascospore germination regulation in the model fungus Podospora anserina

View ORCID ProfileAlexander Demoor, Isabelle Lacaze, View ORCID ProfileRoselyne Ferrari, View ORCID ProfileChristophe Lalanne, View ORCID ProfilePhilippe Silar, View ORCID ProfileSylvain Brun
doi: https://doi.org/10.1101/2022.04.08.487612
Alexander Demoor
Université Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain - UMR 8236, 5 rue Marie-Andrée Lagroua-Weill-Hall, 75205 Paris cedex 13, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Alexander Demoor
Isabelle Lacaze
Université Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain - UMR 8236, 5 rue Marie-Andrée Lagroua-Weill-Hall, 75205 Paris cedex 13, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Roselyne Ferrari
Université Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain - UMR 8236, 5 rue Marie-Andrée Lagroua-Weill-Hall, 75205 Paris cedex 13, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Roselyne Ferrari
Christophe Lalanne
Université Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain - UMR 8236, 5 rue Marie-Andrée Lagroua-Weill-Hall, 75205 Paris cedex 13, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Christophe Lalanne
Philippe Silar
Université Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain - UMR 8236, 5 rue Marie-Andrée Lagroua-Weill-Hall, 75205 Paris cedex 13, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Philippe Silar
Sylvain Brun
Université Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain - UMR 8236, 5 rue Marie-Andrée Lagroua-Weill-Hall, 75205 Paris cedex 13, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sylvain Brun
  • For correspondence: sylvain.brun@u-paris.fr
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

In Podospora anserina as in many other ascomycetes, ascospore germination is a regulated process that requires breaking of dormancy. Despite its importance in survival and dispersal, ascospore germination in filamentous fungi has been poorly investigated and little is known about its regulation and genetic control. We have designed a positive genetic screen that led to the isolation of mutants showing uncontrolled germination, the GUN mutants. In this paper, we report on the characterization of GUN1SG. We show that GUN1SG is mutated in Pa_6_1340, the ortholog of Magnaporthe oryzae Pth2, which encodes a Carnitine-acetyltransferase (CAT) involved in the shuttling of acetyl-CoA between peroxisomes and mitochondria and which is required for appressorium-development. Bioinformatic analysis revealed that the mutated residue (I441) is highly conserved among the Fungi, and that the mutation has a deleterious impact on the protein function. We show that GUN1 is essential for ascospore germination and that the protein is localized both in mitochondria and in peroxisomes. Finally, epistasis studies allowed us to place GUN1 upstream of the PaMpk2 MAPK pathway and the PaNox2/PaPls1 complex in the regulation of ascospore germination. The identification of GUN1, the ortholog of Pth2, in ascospore germination, strengthens the idea of a common genetic regulation governing both appressorium development and melanized ascospore germination. In addition, we characterize the second CAT encoded in P. anserina genome, Pa_3_7660/GUP1, and we show that the function of both CATs is conserved in P. anserina.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted April 10, 2022.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
The GUN mutants: new weapons to unravel ascospore germination regulation in the model fungus Podospora anserina
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
The GUN mutants: new weapons to unravel ascospore germination regulation in the model fungus Podospora anserina
Alexander Demoor, Isabelle Lacaze, Roselyne Ferrari, Christophe Lalanne, Philippe Silar, Sylvain Brun
bioRxiv 2022.04.08.487612; doi: https://doi.org/10.1101/2022.04.08.487612
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
The GUN mutants: new weapons to unravel ascospore germination regulation in the model fungus Podospora anserina
Alexander Demoor, Isabelle Lacaze, Roselyne Ferrari, Christophe Lalanne, Philippe Silar, Sylvain Brun
bioRxiv 2022.04.08.487612; doi: https://doi.org/10.1101/2022.04.08.487612

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genetics
Subject Areas
All Articles
  • Animal Behavior and Cognition (4399)
  • Biochemistry (9637)
  • Bioengineering (7127)
  • Bioinformatics (24959)
  • Biophysics (12677)
  • Cancer Biology (10002)
  • Cell Biology (14406)
  • Clinical Trials (138)
  • Developmental Biology (7992)
  • Ecology (12154)
  • Epidemiology (2067)
  • Evolutionary Biology (16030)
  • Genetics (10957)
  • Genomics (14784)
  • Immunology (9911)
  • Microbiology (23750)
  • Molecular Biology (9516)
  • Neuroscience (51103)
  • Paleontology (370)
  • Pathology (1546)
  • Pharmacology and Toxicology (2694)
  • Physiology (4038)
  • Plant Biology (8700)
  • Scientific Communication and Education (1512)
  • Synthetic Biology (2406)
  • Systems Biology (6461)
  • Zoology (1350)