Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation

Mariano Gonzalez Pisfil; Iliya Nadelson; Brigitte Bergner; Sonja Rottmeier; Andreas W. Thomae; Steffen Dietzel

doi:10.1101/2022.04.13.487856

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Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation

View ORCID ProfileMariano Gonzalez Pisfil, View ORCID ProfileIliya Nadelson, Brigitte Bergner, Sonja Rottmeier, View ORCID ProfileAndreas W. Thomae, View ORCID ProfileSteffen Dietzel

doi: https://doi.org/10.1101/2022.04.13.487856

Mariano Gonzalez Pisfil

1Core Facility Bioimaging and Walter-Brendel-Centre of Experimental Medicine, Biomedical Center, Ludwig-Maximilians-Universität München, Großhaderner Straße 9, 82152 Planegg-Martinsried

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Iliya Nadelson

1Core Facility Bioimaging and Walter-Brendel-Centre of Experimental Medicine, Biomedical Center, Ludwig-Maximilians-Universität München, Großhaderner Straße 9, 82152 Planegg-Martinsried

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Brigitte Bergner

1Core Facility Bioimaging and Walter-Brendel-Centre of Experimental Medicine, Biomedical Center, Ludwig-Maximilians-Universität München, Großhaderner Straße 9, 82152 Planegg-Martinsried

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Sonja Rottmeier

1Core Facility Bioimaging and Walter-Brendel-Centre of Experimental Medicine, Biomedical Center, Ludwig-Maximilians-Universität München, Großhaderner Straße 9, 82152 Planegg-Martinsried

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Andreas W. Thomae

1Core Facility Bioimaging and Walter-Brendel-Centre of Experimental Medicine, Biomedical Center, Ludwig-Maximilians-Universität München, Großhaderner Straße 9, 82152 Planegg-Martinsried

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Steffen Dietzel

1Core Facility Bioimaging and Walter-Brendel-Centre of Experimental Medicine, Biomedical Center, Ludwig-Maximilians-Universität München, Großhaderner Straße 9, 82152 Planegg-Martinsried

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For correspondence: dietzel@lmu.de

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Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation

Mariano Gonzalez Pisfil, Iliya Nadelson, Brigitte Bergner, Sonja Rottmeier, Andreas W. Thomae, Steffen Dietzel

bioRxiv 2022.04.13.487856; doi: https://doi.org/10.1101/2022.04.13.487856

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Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation

Mariano Gonzalez Pisfil, Iliya Nadelson, Brigitte Bergner, Sonja Rottmeier, Andreas W. Thomae, Steffen Dietzel

bioRxiv 2022.04.13.487856; doi: https://doi.org/10.1101/2022.04.13.487856

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