Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Mutations in DNA polymerase δ subunit 1 mediate CMD2-type resistance to Cassava Mosaic Geminiviruses

View ORCID ProfileY.W. Lim, View ORCID ProfileB.N. Mansfeld, View ORCID ProfileP. Schläpfer, View ORCID ProfileK.B. Gilbert, View ORCID ProfileN.N. Narayanan, View ORCID ProfileW. Qi, View ORCID ProfileQ. Wang, View ORCID ProfileZ. Zhong, View ORCID ProfileA. Boyher, J. Gehan, View ORCID ProfileG. Beyene, Z.D. Lin, View ORCID ProfileW. Esuma, View ORCID ProfileS. Feng, View ORCID ProfileC. Chanez, View ORCID ProfileN. Eggenberger, G. Adiga, T. Alicai, S.E. Jacobsen, View ORCID ProfileN.J Taylor, View ORCID ProfileW. Gruissem, View ORCID ProfileR.S. Bart
doi: https://doi.org/10.1101/2022.04.13.487913
Y.W. Lim
1Institute of Molecular Plant Biology, Department of Biology, ETH Zürich, Universitätsstrasse 2, 8092 Zürich, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Y.W. Lim
B.N. Mansfeld
2Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for B.N. Mansfeld
P. Schläpfer
1Institute of Molecular Plant Biology, Department of Biology, ETH Zürich, Universitätsstrasse 2, 8092 Zürich, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for P. Schläpfer
K.B. Gilbert
2Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for K.B. Gilbert
N.N. Narayanan
2Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for N.N. Narayanan
W. Qi
3Functional Genomics Center Zurich, ETH Zurich and University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for W. Qi
Q. Wang
2Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Q. Wang
Z. Zhong
4Department of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Z. Zhong
A. Boyher
2Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for A. Boyher
J. Gehan
2Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
G. Beyene
2Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for G. Beyene
Z.D. Lin
2Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
W. Esuma
5Root Crops Program, National Crops Resources Research Institute, P. O. Box 7084, Kampala, Uganda
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for W. Esuma
S. Feng
4Department of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for S. Feng
C. Chanez
1Institute of Molecular Plant Biology, Department of Biology, ETH Zürich, Universitätsstrasse 2, 8092 Zürich, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for C. Chanez
N. Eggenberger
1Institute of Molecular Plant Biology, Department of Biology, ETH Zürich, Universitätsstrasse 2, 8092 Zürich, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for N. Eggenberger
G. Adiga
5Root Crops Program, National Crops Resources Research Institute, P. O. Box 7084, Kampala, Uganda
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
T. Alicai
5Root Crops Program, National Crops Resources Research Institute, P. O. Box 7084, Kampala, Uganda
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
S.E. Jacobsen
4Department of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA, USA
6Howard Hughes Medical Institute University of California Los Angeles, Los Angeles, CA,USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
N.J Taylor
2Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for N.J Taylor
W. Gruissem
1Institute of Molecular Plant Biology, Department of Biology, ETH Zürich, Universitätsstrasse 2, 8092 Zürich, Switzerland
7Biotechnology Center, National Chung Hsing University, 145 Xingda Road, Taichung City 40227, Taiwan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for W. Gruissem
  • For correspondence: wilhelm_gruissem@ethz.ch RBart@danforthcenter.org
R.S. Bart
2Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for R.S. Bart
  • For correspondence: wilhelm_gruissem@ethz.ch RBart@danforthcenter.org
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

ABSTRACT

Cassava mosaic disease suppresses cassava yields across the tropics. The dominant CMD2 locus confers resistance to the cassava mosaic geminiviruses. It has been reported that CMD2-type landraces lose resistance after regeneration through de novo morphogenesis. As full genome bisulfite sequencing failed to uncover an epigenetic mechanism for loss of resistance, we performed whole genome sequencing and genetic variant analysis and fine-mapped the CMD2 locus to a 190 kilobase interval. Data suggest that CMD2-type resistance is caused by a nonsynonymous, single nucleotide polymorphism in DNA polymerase δ subunit 1 (MePOLD1) located within this region. Virus-induced gene silencing of MePOLD1 in a Cassava mosaic disease-susceptible cassava variety produced a recovery phenotype typical of CMD2-type resistance. Analysis of other CMD2-type cassava varieties identified additional resistance alleles within MePOLD1. MePOLD1 resistance alleles represent important genetic resources for resistance breeding or genome editing, and elucidating mechanisms of resistance to geminiviruses.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted April 13, 2022.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Mutations in DNA polymerase δ subunit 1 mediate CMD2-type resistance to Cassava Mosaic Geminiviruses
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Mutations in DNA polymerase δ subunit 1 mediate CMD2-type resistance to Cassava Mosaic Geminiviruses
Y.W. Lim, B.N. Mansfeld, P. Schläpfer, K.B. Gilbert, N.N. Narayanan, W. Qi, Q. Wang, Z. Zhong, A. Boyher, J. Gehan, G. Beyene, Z.D. Lin, W. Esuma, S. Feng, C. Chanez, N. Eggenberger, G. Adiga, T. Alicai, S.E. Jacobsen, N.J Taylor, W. Gruissem, R.S. Bart
bioRxiv 2022.04.13.487913; doi: https://doi.org/10.1101/2022.04.13.487913
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Mutations in DNA polymerase δ subunit 1 mediate CMD2-type resistance to Cassava Mosaic Geminiviruses
Y.W. Lim, B.N. Mansfeld, P. Schläpfer, K.B. Gilbert, N.N. Narayanan, W. Qi, Q. Wang, Z. Zhong, A. Boyher, J. Gehan, G. Beyene, Z.D. Lin, W. Esuma, S. Feng, C. Chanez, N. Eggenberger, G. Adiga, T. Alicai, S.E. Jacobsen, N.J Taylor, W. Gruissem, R.S. Bart
bioRxiv 2022.04.13.487913; doi: https://doi.org/10.1101/2022.04.13.487913

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Plant Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3603)
  • Biochemistry (7570)
  • Bioengineering (5526)
  • Bioinformatics (20798)
  • Biophysics (10329)
  • Cancer Biology (7985)
  • Cell Biology (11640)
  • Clinical Trials (138)
  • Developmental Biology (6606)
  • Ecology (10205)
  • Epidemiology (2065)
  • Evolutionary Biology (13620)
  • Genetics (9542)
  • Genomics (12847)
  • Immunology (7921)
  • Microbiology (19543)
  • Molecular Biology (7660)
  • Neuroscience (42113)
  • Paleontology (308)
  • Pathology (1258)
  • Pharmacology and Toxicology (2202)
  • Physiology (3267)
  • Plant Biology (7042)
  • Scientific Communication and Education (1294)
  • Synthetic Biology (1951)
  • Systems Biology (5426)
  • Zoology (1117)