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Autophagy modulates apical growth and development in the moss Physcomitrium patens

Georgina Pettinari, Juan Finello, Macarena Plaza Rojas, Franco Liberatore, Germán Robert, View ORCID ProfileSantiago Otaiza-González, Pilar Velez, View ORCID ProfileMartin Theumer, View ORCID ProfilePatricia Agudelo-Romero, Claudio Gonzalez, View ORCID ProfileRamiro Lascano, View ORCID ProfileLaura Saavedra
doi: https://doi.org/10.1101/2022.04.19.488653
Georgina Pettinari
1Unidad Ejecutora de Doble Dependencia INTA-CONICET (UDEA), Córdoba, Argentina
2Cátedra de Fisiología Vegetal, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba; Córdoba, Argentina
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Juan Finello
2Cátedra de Fisiología Vegetal, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba; Córdoba, Argentina
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Macarena Plaza Rojas
2Cátedra de Fisiología Vegetal, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba; Córdoba, Argentina
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Franco Liberatore
1Unidad Ejecutora de Doble Dependencia INTA-CONICET (UDEA), Córdoba, Argentina
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Germán Robert
1Unidad Ejecutora de Doble Dependencia INTA-CONICET (UDEA), Córdoba, Argentina
2Cátedra de Fisiología Vegetal, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba; Córdoba, Argentina
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Santiago Otaiza-González
3Instituto de Investigación Médica Mercedes y Martín Ferreyra - INIMEC (CONICET) - Universidad Nacional de Córdoba, Córdoba, Argentina.
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Pilar Velez
4Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Universidad Nacional de Córdoba-CONICET, Córdoba, Argentina
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Martin Theumer
4Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Universidad Nacional de Córdoba-CONICET, Córdoba, Argentina
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Patricia Agudelo-Romero
5Telethon Kids Institute, Perth Children’s Hospital, Nedlands WA 6009, Australia
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  • ORCID record for Patricia Agudelo-Romero
Claudio Gonzalez
2Cátedra de Fisiología Vegetal, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba; Córdoba, Argentina
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Ramiro Lascano
1Unidad Ejecutora de Doble Dependencia INTA-CONICET (UDEA), Córdoba, Argentina
2Cátedra de Fisiología Vegetal, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba; Córdoba, Argentina
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  • For correspondence: ramiro.lascano@unc.edu.ar
Laura Saavedra
1Unidad Ejecutora de Doble Dependencia INTA-CONICET (UDEA), Córdoba, Argentina
2Cátedra de Fisiología Vegetal, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba; Córdoba, Argentina
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  • For correspondence: ramiro.lascano@unc.edu.ar
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ABSTRACT

Different to root hairs and pollen tubes, Physcomitrium patens apical growing protonemal cells have the singularity that they continue to undergo cell divisions as the plant develops, allowing to study autophagy in the context of a multicellular apical growing tissue coupled to development. Herein, we showed that the core autophagy machinery is present in the moss P. patens, and deeply characterized the growth and development of wild-type, atg5 and atg7 loss-of-function mutants under optimal and nutrient-deprived conditions. Our results showed that the growth of the different morphological and functional protonemata apical growing cells, chloronema and caulonema, is differentially modulated by this process. These differences depend on the protonema cell type and position along the protonemal filament, and growth condition. As a global plant response, the absence of autophagy triggers the spread of the colony through protonemata growth at the expense of a reduction in buds and gametophore development, and thus the adult gametophytic and reproductive phases. Altogether this study provides valuable information indicating that autophagy has roles during apical growth with differential responses within the cell types of the same tissue and contributes to life cycle progression and thus the development of the 2D and 3D tissues of P. patens.

HIGHLIGHT Autophagy is differentially induced in protonemal cells, and contributes to apical growth, life cycle progression, and thus the development of the 2D and 3D tissues of P. patens.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Georgina Pettinari, E-mail: pettinari.georgina{at}inta.gob.ar

    Juan Finello, E-mail: juanfinello{at}gmail.com

    Macarena Plaza Rojas, E-mail: makuplazarojas{at}gmail.com

    Franco Liberatore, E-mail: frliberatore{at}gmail.com

    Germán Robert, E-mail: robert.german{at}inta.gob.ar

    Santiago Otaiza-González, E-mail: santiagootaiza{at}unc.edu.ar

    Pilar Velez, E-mail: pilar.velez{at}unc.edu.ar

    Martin Theumer, E-mail: mgtheumer{at}unc.edu.ar

    Patricia Agudelo-Romero, E-mail: Patricia.AgudeloRomero{at}telethonkids.org.au

    Claudio Gonzalez, E-mail: Cgonzalez695{at}unc.edu.ar

    Ramiro Lascano, E-mail: ramiro.lascano{at}unc.edu.ar

    Laura Saavedra, E-mail: laura.saavedra{at}conicet.gov.ar

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted April 19, 2022.
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Autophagy modulates apical growth and development in the moss Physcomitrium patens
Georgina Pettinari, Juan Finello, Macarena Plaza Rojas, Franco Liberatore, Germán Robert, Santiago Otaiza-González, Pilar Velez, Martin Theumer, Patricia Agudelo-Romero, Claudio Gonzalez, Ramiro Lascano, Laura Saavedra
bioRxiv 2022.04.19.488653; doi: https://doi.org/10.1101/2022.04.19.488653
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Autophagy modulates apical growth and development in the moss Physcomitrium patens
Georgina Pettinari, Juan Finello, Macarena Plaza Rojas, Franco Liberatore, Germán Robert, Santiago Otaiza-González, Pilar Velez, Martin Theumer, Patricia Agudelo-Romero, Claudio Gonzalez, Ramiro Lascano, Laura Saavedra
bioRxiv 2022.04.19.488653; doi: https://doi.org/10.1101/2022.04.19.488653

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