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History
- April 20, 2022.
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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Author Information
- George-Rafael Samantsidis1,2,
- Melina Fotiadou2,
- Savvas Tzavellas2,
- Sven Geibel3,
- Ralf Nauen3,
- Luc Swevers4,
- Shane Denecke1,5,*,& and
- John Vontas1,6,*,&
- 1Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Greece
- 2Department of Biology, University of Crete, Vassilika Vouton, 71409 Heraklion, Crete, Greece
- 3R&D Pest Control, Bayer AG, Crop Science Division, Monheim, Germany
- 4Insect Molecular Genetics and Biotechnology, National Centre for Scientific Research Demokritos, Institute of Biosciences and Applications, 15310 Athens, Greece
- 6Pesticide Science Lab, Department of Crop Science, Agricultural University of Athens, Greece
- 5Department of Pathobiology, University of Pennsylvania, Philadelphia, United states
- ↵*correspondence: Shane Denecke (sdenecke{at}upenn.edu) or John Vontas (vontas{at}imbb.forth.gr)
↵& equal last authors
Authors’ contribution: S.D and J.V conceived the project, S.D. and G-R.S designed the experiments, G-R.S performed the CRISPR in cell lines and generated stable cells, G-R.S and S.T. performed the luciferase assays in cell cultures, G-R.S performed the gene expression, cell death analysis and immunofluorescence in cell lines, S.D. and M.F. performed the phylogenetic analysis, M.F. performed the CRISPR in S. frugiperda. G-R.S, S.D and J.V analyzed the results. G-R.S, M.F, S.D and J.V. wrote the manuscript. The manuscript has been revised and accepted by all authors.