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Active mechanics of sea star oocytes

View ORCID ProfilePeter J. Foster, View ORCID ProfileSebastian Fürthauer, View ORCID ProfileNikta Fakhri
doi: https://doi.org/10.1101/2022.04.22.489189
Peter J. Foster
1Physics of Living Systems, Department of Physics, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
†Department of Physics, Brandeis University, Waltham, MA, 02454, USA
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  • For correspondence: foster@brandeis.edu fuerthauer@iap.tuwien.ac.at fakhri@mit.edu
Sebastian Fürthauer
2Institute for Applied Physics, TU Wien, A-1040 Wien, Austria
3Center for Computational Biology, Flatiron Institute, New York, NY, 10010, USA
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  • For correspondence: foster@brandeis.edu fuerthauer@iap.tuwien.ac.at fakhri@mit.edu
Nikta Fakhri
1Physics of Living Systems, Department of Physics, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
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  • For correspondence: foster@brandeis.edu fuerthauer@iap.tuwien.ac.at fakhri@mit.edu
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Abstract

Actomyosin is a canonical example of an active material, driven out of equilibrium in part through the injection of energy by myosin motors. This influx of energy allows actomyosin networks to generate cellular-scale contractility, which underlies cellular processes ranging from division to migration. While the molecular players underlying actomyosin contractility have been well characterized, how cellular-scale deformation in disordered actomyosin networks emerges from filament-scale interactions is not well understood. Here, we address this question in vivo using the meiotic surface contraction wave of Patiria miniata oocytes. Using pharmacological treatments targeting actin polymerization, we find that the cellular deformation rate is a nonmonotonic function of cortical actin density peaked near the wild type density. To understand this, we develop an active fluid model coarse-grained from filament-scale interactions and find quantitative agreement with the measured data. This model further predicts the dependence of the deformation rate on the concentration of passive actin crosslinkers and motor proteins, including the surprising prediction that deformation rate decreases with increasing motor concentration. We test these predictions through protein overexpression and find quantitative agreement. Taken together, this work is an important step for bridging the molecular and cellular length scales for cytoskeletal networks in vivo.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted April 22, 2022.
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Active mechanics of sea star oocytes
Peter J. Foster, Sebastian Fürthauer, Nikta Fakhri
bioRxiv 2022.04.22.489189; doi: https://doi.org/10.1101/2022.04.22.489189
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Active mechanics of sea star oocytes
Peter J. Foster, Sebastian Fürthauer, Nikta Fakhri
bioRxiv 2022.04.22.489189; doi: https://doi.org/10.1101/2022.04.22.489189

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