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Salivary Proinflammatory Cytokines IL-1β, IL-6 and TNF Decrease With Age

Evangelina Costantino, Sofía Daiana Castell, María Florencia Harman, María Cristina Pistoresi- Palencia, Adriana Beatriz Actis
doi: https://doi.org/10.1101/2022.04.25.489450
Evangelina Costantino
1Cátedra B de Anatomía, Facultad de Odontología, Universidad Nacional de Córdoba. Haya de la Torre s/n. Ciudad Universitaria. 5000 Córdoba, Argentina
2Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET y Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Blvd. de la Reforma s/n. Ciudad Universitaria. 5000 Córdoba. Argentina
PhD.
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Sofía Daiana Castell
3Centro de Investigación en Bioquímica Clínica e Inmunología, Consejo Nacional de Investigaciones Científicas y Técnicas, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba. Haya de la Torre y Avenida Medina Allende. Ciudad Universitaria. 5000 Córdoba, Argentina
PhD.
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María Florencia Harman
3Centro de Investigación en Bioquímica Clínica e Inmunología, Consejo Nacional de Investigaciones Científicas y Técnicas, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba. Haya de la Torre y Avenida Medina Allende. Ciudad Universitaria. 5000 Córdoba, Argentina
PhD.
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María Cristina Pistoresi- Palencia
3Centro de Investigación en Bioquímica Clínica e Inmunología, Consejo Nacional de Investigaciones Científicas y Técnicas, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba. Haya de la Torre y Avenida Medina Allende. Ciudad Universitaria. 5000 Córdoba, Argentina
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Adriana Beatriz Actis
4Instituto de Investigaciones en Ciencias de la Salud (INICSA), Consejo Nacional de Investigaciones Científicas y Técnicas y Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Blvd. de la Reforma s/n. Ciudad Universitaria. 5000 Córdoba. Argentina
PhD.
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  • For correspondence: adriana.actis@unc.edu.ar
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ABSTRACT

Objective to analyze salivary concentrations interleukin-1β (IL-1β), 6 (IL-6) and tumor necrosis factor (TNF) according to age in healthy subjects to determine baseline inflammatory state of the oral mucosa in elderly subjects, who are at higher risk of developing inflammation-related diseases.

Materials and methods sixty-four healthy volunteers were divided into groups according to age: 20-39 (G1; n=20); 40-59 (G2; n=24); 60-80 years (G3; n=20). Their stomatognathic system and periodontal status were assessed; consumption of food sources of fatty acids (FA) was evaluated using a validated food frequency questionnaire. IL-1β, IL-6, and TNF salivary levels were determined (ELISA) in mixed unstimulated saliva. Kruskal Wallis and Spearman’s correlation tests were applied (p<0.05).

Results salivary IL-1β and TNF levels were lower in G2 (p=0.001) and G3 (p<0.001) than in G1; IL-6 levels were lower in G3 than in G2 and G1 (p= <0.001). IL-1β showed the highest concentration in all groups (p<0.001). No statistically significant differences in salivary cytokine levels were observed on sex in any of the groups. Associations were observed between salivary cytokines and consumption of some foods containing FA.

Conclusion salivary concentration of proinflammatory cytokines decreased with age. It could be influenced by ageing-related changes. In addtition, the baseline inflammatory state of the oral mucosa would appear to be influenced by dietary intake of sources of anti-inflammatory FA.

Clinical relevance the knowledge about inflammatory state of the oral mucosa, as determined by salivary cytokine concentrations, could be useful for understanding and prevention of developing inflammation-related diseases.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • E-mail address: evangelina.costantino{at}unc.edu.ar, Fax number: +54 351 4334178. Telephone number: +54 351 4334274, int. 157.

  • E-mail address: scastell{at}unc.edu.ar

  • E-mail address: mharman{at}fcq.unc.edu.ar

  • E-mail address: mcpistoresi{at}gmail.com

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted April 26, 2022.
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Salivary Proinflammatory Cytokines IL-1β, IL-6 and TNF Decrease With Age
Evangelina Costantino, Sofía Daiana Castell, María Florencia Harman, María Cristina Pistoresi- Palencia, Adriana Beatriz Actis
bioRxiv 2022.04.25.489450; doi: https://doi.org/10.1101/2022.04.25.489450
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Salivary Proinflammatory Cytokines IL-1β, IL-6 and TNF Decrease With Age
Evangelina Costantino, Sofía Daiana Castell, María Florencia Harman, María Cristina Pistoresi- Palencia, Adriana Beatriz Actis
bioRxiv 2022.04.25.489450; doi: https://doi.org/10.1101/2022.04.25.489450

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