Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

The Drosophila Fab-7 boundary element modulates Abd-B gene activity in the genital disc by guiding an inversion of collinear chromatin organization and alternative promoter use

Laura Moniot-Perron, View ORCID ProfileBenoit Moindrot, View ORCID ProfileLine Manceau, Joanne Edouard, View ORCID ProfileYan Jaszczyszyn, View ORCID ProfilePascale Gilardi-Hebenstreit, View ORCID ProfileCéline Hernandez, Sébastien Bloyer, View ORCID ProfileDaan Noordermeer
doi: https://doi.org/10.1101/2022.04.26.489596
Laura Moniot-Perron
1Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France
3European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Benoit Moindrot
1Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Benoit Moindrot
Line Manceau
2Université Paris Cité, CNRS, Institut Jacques Monod, Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Line Manceau
Joanne Edouard
1Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yan Jaszczyszyn
1Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Yan Jaszczyszyn
Pascale Gilardi-Hebenstreit
2Université Paris Cité, CNRS, Institut Jacques Monod, Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Pascale Gilardi-Hebenstreit
Céline Hernandez
1Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Céline Hernandez
Sébastien Bloyer
1Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: sebastien.bloyer@i2bc.paris-saclay.fr daan.noordermeer@i2bc.paris-saclay.fr
Daan Noordermeer
1Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Daan Noordermeer
  • For correspondence: sebastien.bloyer@i2bc.paris-saclay.fr daan.noordermeer@i2bc.paris-saclay.fr
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Summary

Hox genes encode transcription factors that specify segmental identities along the Antero-Posterior body axis. These genes are organized in clusters, where their order corresponds to their activity along the body axis, an evolutionary conserved feature known as collinearity. In Drosophila, the BX-C cluster contains the three most posterior Hox genes, where their collinear activation incorporates progressive replacement of histone modifications, reorganization of 3D chromatin architecture and sequential activation of boundary elements and cis-regulatory regions. To dissect functional hierarchies, we compared chromatin organization in larvae and in cell lines, with a focus on the Abd-B gene. Our work establishes the importance of the Fab-7 boundary element for insulation between 3D domains marked by different histone modifications. Interestingly, we detected a non-canonical inversion of collinear chromatin dynamics at the Abd-B gene, with the active histone domain decreasing in size. This chromatin organization differentially instructed alternative Abd-B promoter use, thereby expanding the possibilities to regulate transcriptional output.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
Back to top
PreviousNext
Posted April 26, 2022.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
The Drosophila Fab-7 boundary element modulates Abd-B gene activity in the genital disc by guiding an inversion of collinear chromatin organization and alternative promoter use
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
The Drosophila Fab-7 boundary element modulates Abd-B gene activity in the genital disc by guiding an inversion of collinear chromatin organization and alternative promoter use
Laura Moniot-Perron, Benoit Moindrot, Line Manceau, Joanne Edouard, Yan Jaszczyszyn, Pascale Gilardi-Hebenstreit, Céline Hernandez, Sébastien Bloyer, Daan Noordermeer
bioRxiv 2022.04.26.489596; doi: https://doi.org/10.1101/2022.04.26.489596
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
The Drosophila Fab-7 boundary element modulates Abd-B gene activity in the genital disc by guiding an inversion of collinear chromatin organization and alternative promoter use
Laura Moniot-Perron, Benoit Moindrot, Line Manceau, Joanne Edouard, Yan Jaszczyszyn, Pascale Gilardi-Hebenstreit, Céline Hernandez, Sébastien Bloyer, Daan Noordermeer
bioRxiv 2022.04.26.489596; doi: https://doi.org/10.1101/2022.04.26.489596

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Developmental Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4237)
  • Biochemistry (9155)
  • Bioengineering (6797)
  • Bioinformatics (24052)
  • Biophysics (12149)
  • Cancer Biology (9562)
  • Cell Biology (13814)
  • Clinical Trials (138)
  • Developmental Biology (7653)
  • Ecology (11729)
  • Epidemiology (2066)
  • Evolutionary Biology (15534)
  • Genetics (10663)
  • Genomics (14346)
  • Immunology (9503)
  • Microbiology (22876)
  • Molecular Biology (9113)
  • Neuroscience (49080)
  • Paleontology (357)
  • Pathology (1487)
  • Pharmacology and Toxicology (2576)
  • Physiology (3851)
  • Plant Biology (8347)
  • Scientific Communication and Education (1473)
  • Synthetic Biology (2299)
  • Systems Biology (6202)
  • Zoology (1302)