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Removal of Clinically Relevant SARS-CoV-2 Variants by An Affinity Resin Containing Galanthus nivalis Agglutinin

Melanie Gooldy, Christelle M. Roux, View ORCID ProfileSteven P. LaRosa, Nicole Spaulding, Charles J. Fisher Jr.
doi: https://doi.org/10.1101/2022.04.27.489436
Melanie Gooldy
1CUBRC, Inc., Buffalo, NY
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Christelle M. Roux
1CUBRC, Inc., Buffalo, NY
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Steven P. LaRosa
2Aethlon Medical, Inc., San Diego, CA
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  • For correspondence: slarosamd@aethlonmedical.con
Nicole Spaulding
1CUBRC, Inc., Buffalo, NY
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Charles J. Fisher Jr.
2Aethlon Medical, Inc., San Diego, CA
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Abstract

The Coronavirus-19 (COVID-19) pandemic due to the SARS-CoV-2 virus has now exceeded two years in duration. The pandemic has been characterized by the development of a succession of variants containing mutations in the spike protein affecting infectiousness, virulence and efficacy of vaccines and monoclonal antibodies. Resistance to vaccination and limitations in the current treatments available require the ongoing development of therapies especially for those with severe disease. The plant lectin Galanthus nivalis binds to mannose structures in the viral envelope. We hypothesized that viral binding should be unaffected by spike protein mutations. Known concentrations of seven clinically relevant SARS-CoV-2 variants were spiked in medium and passed three times over columns containing 1 gm of GNA affinity resin. Percent decrease in viral titer was compared with a control sample. Viral capture efficiency was found to range from 53 to 89% for all variants. Extrapolation indicated that an adult Aethlon Hemopurifier® would have more than sufficient binding capacity for viral loads observed in adult patients with severe COVID-19 infection.

Competing Interest Statement

This study was funded by Aethlon Medical, Inc. Aethlon Medical provided the materials and GNA affinity resin to perform the study. Authors SPL and CSF are employees of Aethlon Medical, Inc.

Footnotes

  • Financial Support: This study was funded by Aethlon Medical, Inc. Aethlon Medical provided the materials and GNA affinity resin to perform the study.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted April 28, 2022.
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Removal of Clinically Relevant SARS-CoV-2 Variants by An Affinity Resin Containing Galanthus nivalis Agglutinin
Melanie Gooldy, Christelle M. Roux, Steven P. LaRosa, Nicole Spaulding, Charles J. Fisher Jr.
bioRxiv 2022.04.27.489436; doi: https://doi.org/10.1101/2022.04.27.489436
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Removal of Clinically Relevant SARS-CoV-2 Variants by An Affinity Resin Containing Galanthus nivalis Agglutinin
Melanie Gooldy, Christelle M. Roux, Steven P. LaRosa, Nicole Spaulding, Charles J. Fisher Jr.
bioRxiv 2022.04.27.489436; doi: https://doi.org/10.1101/2022.04.27.489436

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