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Joint effect of temperature and insect chitosan on the heat resistance of Bacillus cereus spores in rice derivatives

Maria Ines Valdez, View ORCID ProfileUbeda Maria, Narvaes Cristian, View ORCID ProfileRodrigo Dolores, Martinez Antonio
doi: https://doi.org/10.1101/2022.04.28.489901
Maria Ines Valdez
Instituto de Agroquímica y Tecnología de Alimentos (CSIC). Agustín Escardino 10. 46980. Paterna. Valencia
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Ubeda Maria
Instituto de Agroquímica y Tecnología de Alimentos (CSIC). Agustín Escardino 10. 46980. Paterna. Valencia
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  • ORCID record for Ubeda Maria
Narvaes Cristian
Instituto de Agroquímica y Tecnología de Alimentos (CSIC). Agustín Escardino 10. 46980. Paterna. Valencia
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Rodrigo Dolores
Instituto de Agroquímica y Tecnología de Alimentos (CSIC). Agustín Escardino 10. 46980. Paterna. Valencia
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Martinez Antonio
Instituto de Agroquímica y Tecnología de Alimentos (CSIC). Agustín Escardino 10. 46980. Paterna. Valencia
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Summary

The heat resistance of Bacillus cereus spores inoculated in a rice substrate supplemented with insect chitosan as an alternative antimicrobial was studied. Two concentrations of insect chitosan were considered in order to assess the role of the insect chitosan concentration during the heat process as an antimicrobial replacement for crustacean chitosan.

Results of the study indicated that the DT values were clearly higher in the substrate without chitosan than in the substrate containing chitosan thus indicating a greater heat resistance to heat treatment of the microorganism inoculated in the substrate without chitosan. This behaviour was also evidenced in the survival curves. There were no great differences between either of the insect chitosan concentrations tested regarding the DT values. The z values were 9.8 ºC on rice substrate. 8.9 ºC on rice substrate supplemented with insect chitosan at 150 µg/mL and 10.7 ºC on rice substrate supplemented with 250 µg/mL of insect chitosan, the chitosan concentration appears to affect the z value of the microorganism. Our results indicate that the combination of heat with insect chitosan as an antimicrobial on foodstuffs subjected to cooking is feasible and can improve the safety of rice derivatives.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted April 28, 2022.
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Joint effect of temperature and insect chitosan on the heat resistance of Bacillus cereus spores in rice derivatives
Maria Ines Valdez, Ubeda Maria, Narvaes Cristian, Rodrigo Dolores, Martinez Antonio
bioRxiv 2022.04.28.489901; doi: https://doi.org/10.1101/2022.04.28.489901
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Joint effect of temperature and insect chitosan on the heat resistance of Bacillus cereus spores in rice derivatives
Maria Ines Valdez, Ubeda Maria, Narvaes Cristian, Rodrigo Dolores, Martinez Antonio
bioRxiv 2022.04.28.489901; doi: https://doi.org/10.1101/2022.04.28.489901

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