Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Detergent headgroups control TolC folding in vitro

Ayotunde Paul Ikujuni, S. Jimmy Budiardjo, Joanna S.G. Slusky
doi: https://doi.org/10.1101/2022.04.28.489915
Ayotunde Paul Ikujuni
aDepartment of Molecular Biosciences, The University of Kansas, 1200 Sunnyside Ave, Lawrence, KS 66045
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
S. Jimmy Budiardjo
bCenter for Computational Biology, The University of Kansas, 2030 Becker Dr., Lawrence, KS 66045-7534
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joanna S.G. Slusky
aDepartment of Molecular Biosciences, The University of Kansas, 1200 Sunnyside Ave, Lawrence, KS 66045
bCenter for Computational Biology, The University of Kansas, 2030 Becker Dr., Lawrence, KS 66045-7534
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: slusky@ku.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

TolC is the trimeric outer membrane component of the efflux pump system in E. coli responsible for antibiotic efflux from bacterial cells. Over-expression of efflux pumps has been reported to decrease susceptibility to antibiotics in a variety of bacterial pathogens. Reliable production of membrane proteins allows for the biophysical and structural characterization needed to better understand efflux and for the development of therapeutics. Preparation of recombinant protein for biochemical/structural studies often involves the production of proteins as inclusion body aggregates from which bioactive proteins are recovered. Here we find that the in vitro folding of TolC into its functional trimeric state from inclusion bodies is dependent on the headgroup composition of detergent micelles used. Nonionic detergent favors the formation of functional trimeric TolC, whereas zwitterionic detergents induce the formation of a non-native trimeric TolC fold. We also find that nonionic detergents with shorter alkyl lengths facilitate TolC folding. It remains to be seen whether the charges in lipid headgroups have similar effects on membrane insertion and folding in biological systems.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted April 29, 2022.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Detergent headgroups control TolC folding in vitro
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Detergent headgroups control TolC folding in vitro
Ayotunde Paul Ikujuni, S. Jimmy Budiardjo, Joanna S.G. Slusky
bioRxiv 2022.04.28.489915; doi: https://doi.org/10.1101/2022.04.28.489915
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Detergent headgroups control TolC folding in vitro
Ayotunde Paul Ikujuni, S. Jimmy Budiardjo, Joanna S.G. Slusky
bioRxiv 2022.04.28.489915; doi: https://doi.org/10.1101/2022.04.28.489915

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biophysics
Subject Areas
All Articles
  • Animal Behavior and Cognition (4397)
  • Biochemistry (9623)
  • Bioengineering (7118)
  • Bioinformatics (24928)
  • Biophysics (12658)
  • Cancer Biology (9985)
  • Cell Biology (14395)
  • Clinical Trials (138)
  • Developmental Biology (7985)
  • Ecology (12141)
  • Epidemiology (2067)
  • Evolutionary Biology (16020)
  • Genetics (10947)
  • Genomics (14774)
  • Immunology (9896)
  • Microbiology (23730)
  • Molecular Biology (9501)
  • Neuroscience (51036)
  • Paleontology (370)
  • Pathology (1544)
  • Pharmacology and Toxicology (2690)
  • Physiology (4035)
  • Plant Biology (8687)
  • Scientific Communication and Education (1512)
  • Synthetic Biology (2404)
  • Systems Biology (6453)
  • Zoology (1349)