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Plasma membrane topography governs the three-dimensional dynamic localization of IgM B cell receptor clusters

View ORCID ProfileDeniz Saltukoglu, View ORCID ProfileBugra Özdemir, View ORCID ProfileMichael Holtmannspötter, View ORCID ProfileRalf Reski, View ORCID ProfileJacob Piehler, View ORCID ProfileRainer Kurre, View ORCID ProfileMichael Reth
doi: https://doi.org/10.1101/2022.04.29.489661
Deniz Saltukoglu
1Department of Molecular Immunology, Biology III, Faculty of Biology, University of Freiburg, 79104 Freiburg Germany
2Signaling Research Centers CIBSS and BIOSS, University of Freiburg, 79104 Freiburg, Germany
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Bugra Özdemir
2Signaling Research Centers CIBSS and BIOSS, University of Freiburg, 79104 Freiburg, Germany
3Plant Biotechnology, Faculty of Biology, University of Freiburg, 79104, Freiburg, Germany
5Euro-BioImaging, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
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Michael Holtmannspötter
4Department of Biology/Chemistry and Center for Cellular Nanoanalytics, Osnabrück University, 49076 Osnabrück, Germany
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Ralf Reski
2Signaling Research Centers CIBSS and BIOSS, University of Freiburg, 79104 Freiburg, Germany
3Plant Biotechnology, Faculty of Biology, University of Freiburg, 79104, Freiburg, Germany
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Jacob Piehler
4Department of Biology/Chemistry and Center for Cellular Nanoanalytics, Osnabrück University, 49076 Osnabrück, Germany
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Rainer Kurre
4Department of Biology/Chemistry and Center for Cellular Nanoanalytics, Osnabrück University, 49076 Osnabrück, Germany
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Michael Reth
1Department of Molecular Immunology, Biology III, Faculty of Biology, University of Freiburg, 79104 Freiburg Germany
2Signaling Research Centers CIBSS and BIOSS, University of Freiburg, 79104 Freiburg, Germany
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  • For correspondence: Michael.reth@bioss.uni-freiburg.de
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Abstract

B lymphocytes recognize bacterial or viral antigens via different classes of the B cell antigen receptor (BCR). Protrusive structures termed microvilli cover lymphocyte surfaces and are thought to perform sensory functions in screening antigen-bearing surfaces. Here, we have studied the cell surface features of Ramos B cells and the spatiotemporal organization of the IgM-BCR using lattice light sheet microscopy in combination with tailored custom-built 4D image analysis. Ramos B cell surfaces were found to form dynamic networks of elevated ridges bridging individual microvilli. A proportion of membrane-localized IgM-BCR was found in clusters, which were associated with the ridges and the microvilli. The dynamic ridge network organization and the IgM-BCR cluster mobility were linked and both were controlled by Arp2/3 complex activity. Our results suggest that topographical features of the cell surface govern the distribution and dynamic localization of IgM-BCR clusters to facilitate antigen screening.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted April 29, 2022.
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Plasma membrane topography governs the three-dimensional dynamic localization of IgM B cell receptor clusters
Deniz Saltukoglu, Bugra Özdemir, Michael Holtmannspötter, Ralf Reski, Jacob Piehler, Rainer Kurre, Michael Reth
bioRxiv 2022.04.29.489661; doi: https://doi.org/10.1101/2022.04.29.489661
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Plasma membrane topography governs the three-dimensional dynamic localization of IgM B cell receptor clusters
Deniz Saltukoglu, Bugra Özdemir, Michael Holtmannspötter, Ralf Reski, Jacob Piehler, Rainer Kurre, Michael Reth
bioRxiv 2022.04.29.489661; doi: https://doi.org/10.1101/2022.04.29.489661

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