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High fat diet changes bacterial signatures in the murine pancreas

View ORCID ProfileTorsten P.M. Scheithauer, Mark Davids, Maaike Winkelmeijer, Stefan Havik, Max Nieuwdorp, View ORCID ProfileHilde Herrema, Daniël H. van Raalte
doi: https://doi.org/10.1101/2022.04.29.490014
Torsten P.M. Scheithauer
1Department of Experimental Vascular Medicine, Amsterdam University Medical Center (UMC), location Amsterdam Medical Center (AMC), Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
2Department of Internal Medicine, Amsterdam University Medical Center (UMC), location Vrije University Medical Center (VUmc), The Netherlands
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  • For correspondence: t.p.scheithauer@amsterdamumc.nl
Mark Davids
1Department of Experimental Vascular Medicine, Amsterdam University Medical Center (UMC), location Amsterdam Medical Center (AMC), Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
2Department of Internal Medicine, Amsterdam University Medical Center (UMC), location Vrije University Medical Center (VUmc), The Netherlands
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Maaike Winkelmeijer
1Department of Experimental Vascular Medicine, Amsterdam University Medical Center (UMC), location Amsterdam Medical Center (AMC), Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
2Department of Internal Medicine, Amsterdam University Medical Center (UMC), location Vrije University Medical Center (VUmc), The Netherlands
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Stefan Havik
1Department of Experimental Vascular Medicine, Amsterdam University Medical Center (UMC), location Amsterdam Medical Center (AMC), Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
2Department of Internal Medicine, Amsterdam University Medical Center (UMC), location Vrije University Medical Center (VUmc), The Netherlands
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Max Nieuwdorp
1Department of Experimental Vascular Medicine, Amsterdam University Medical Center (UMC), location Amsterdam Medical Center (AMC), Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
2Department of Internal Medicine, Amsterdam University Medical Center (UMC), location Vrije University Medical Center (VUmc), The Netherlands
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Hilde Herrema
1Department of Experimental Vascular Medicine, Amsterdam University Medical Center (UMC), location Amsterdam Medical Center (AMC), Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
2Department of Internal Medicine, Amsterdam University Medical Center (UMC), location Vrije University Medical Center (VUmc), The Netherlands
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Daniël H. van Raalte
1Department of Experimental Vascular Medicine, Amsterdam University Medical Center (UMC), location Amsterdam Medical Center (AMC), Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
2Department of Internal Medicine, Amsterdam University Medical Center (UMC), location Vrije University Medical Center (VUmc), The Netherlands
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Abstract

Hyperglycemia is caused by failure of pancreatic beta cells. Beta-cell inflammation contributes to beta-cell dysfunction, however (primary) immunogenic triggers are in large unknown. The gut microbiota is one potential source of pro-inflammatory molecules. A high-fat diet treatment increases pro-inflammatory bacteria in the gut microbiome, parts of which could migrate to the pancreatic beta-cell. In the present study, the bacterial DNA signature in the pancreas and intestine of C57BL6/J mice was analyzed. Mice were fed a high-fat diet (60% kcal fat) or a regular chow diet for 12 weeks. We took several precautions to avoid and map contamination. The gut microbiota was affected by high-fat diet as following: We observed several common intestinal ASVs in the pancreatic tissue. Although the pancreatic ASVs do not correlate exactly with the gut ASVs, our data implicate that the pancreas contains bacterial DNA and that this signature is altered in high-fat diet fed mice (PERMONOVA, p = 0.037; betadisper, p=0.029). Gut derived bacterial DNA might end up in the pancreas at some point in time. Hence, this work supports the concept of translocation of bacterial DNA to the pancreas, which might contribute to inflammation and dysfunction of pancreatic beta-cells.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted April 29, 2022.
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High fat diet changes bacterial signatures in the murine pancreas
Torsten P.M. Scheithauer, Mark Davids, Maaike Winkelmeijer, Stefan Havik, Max Nieuwdorp, Hilde Herrema, Daniël H. van Raalte
bioRxiv 2022.04.29.490014; doi: https://doi.org/10.1101/2022.04.29.490014
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High fat diet changes bacterial signatures in the murine pancreas
Torsten P.M. Scheithauer, Mark Davids, Maaike Winkelmeijer, Stefan Havik, Max Nieuwdorp, Hilde Herrema, Daniël H. van Raalte
bioRxiv 2022.04.29.490014; doi: https://doi.org/10.1101/2022.04.29.490014

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