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Different combinations of laccase paralogs non-redundantly control the lignin amount and composition of specific cell types and cell wall layers in Arabidopsis

View ORCID ProfileLeonard Blaschek, Emiko Murozuka, Delphine Ménard, View ORCID ProfileEdouard Pesquet
doi: https://doi.org/10.1101/2022.05.04.490011
Leonard Blaschek
1Arrhenius laboratories, Department of Ecology, Environment and Plant Sciences (DEEP), Svante Arrhenius väg 20A, Stockholm University, 160 91 Stockholm, Sweden
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Emiko Murozuka
1Arrhenius laboratories, Department of Ecology, Environment and Plant Sciences (DEEP), Svante Arrhenius väg 20A, Stockholm University, 160 91 Stockholm, Sweden
2Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University, 901 87 Umeå, Sweden
3Carlsberg Research Laboratory, J.C. Jacobsens Gade 4, DK-1799 Copenhagen V, Denmark
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Delphine Ménard
1Arrhenius laboratories, Department of Ecology, Environment and Plant Sciences (DEEP), Svante Arrhenius väg 20A, Stockholm University, 160 91 Stockholm, Sweden
2Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University, 901 87 Umeå, Sweden
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Edouard Pesquet
1Arrhenius laboratories, Department of Ecology, Environment and Plant Sciences (DEEP), Svante Arrhenius väg 20A, Stockholm University, 160 91 Stockholm, Sweden
2Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University, 901 87 Umeå, Sweden
4Bolin Centre for Climate Research, Stockholm University, 106 91 Stockholm, Sweden
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  • For correspondence: edouard.pesquet@su.se
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Abstract

Vascular plants reinforce the cell walls of the different xylem cell types with lignin, a phenolic polymer. Specific lignin chemistries are conserved between the cell wall layers of each cell type to support their functions. Yet the mechanisms controlling the tight spatial localisation of specific lignin chemistries remain unclear. Current hypotheses focus on a control by monomer biosynthesis and/or export, while their cell wall polymerisation is viewed as random and non-limiting. Here we show that cell wall polymerisation using combinations of multiple different laccases (LACs) non-redundantly and specifically control the lignin chemistry in different cell types and their distinct cell wall layers. We dissected the roles of Arabidopsis thaliana LAC4, 5, 10, 12 and 17 by generating quadruple and quintuple loss-of-function mutants. Different combinatory loss of these LACs lead to specific changes in lignin chemistry affecting both residue ring structures and/or aliphatic tails in specific cell types and cell wall layers. We moreover showed that the LAC-mediated lignification had distinct functions in specific cell types. Altogether, we propose that the spatial control of lignin chemistry depends on different combinations of LACs with non-redundant activities immobilised in specific cell types and cell wall layers.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted May 05, 2022.
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Different combinations of laccase paralogs non-redundantly control the lignin amount and composition of specific cell types and cell wall layers in Arabidopsis
Leonard Blaschek, Emiko Murozuka, Delphine Ménard, Edouard Pesquet
bioRxiv 2022.05.04.490011; doi: https://doi.org/10.1101/2022.05.04.490011
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Different combinations of laccase paralogs non-redundantly control the lignin amount and composition of specific cell types and cell wall layers in Arabidopsis
Leonard Blaschek, Emiko Murozuka, Delphine Ménard, Edouard Pesquet
bioRxiv 2022.05.04.490011; doi: https://doi.org/10.1101/2022.05.04.490011

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