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The effect of dietary chitin on Atlantic salmon (Salmo salar) chitinase activity, gene expression, and microbial composition

Matilde Mengkrog Holen, View ORCID ProfileSimen Rød Sandve, View ORCID ProfileThomas Nelson Harvey, View ORCID ProfileYang Jin, View ORCID ProfileInga Leena Angell, View ORCID ProfileKnut Rudi, View ORCID ProfileMatthew Peter Kent
doi: https://doi.org/10.1101/2022.05.05.490722
Matilde Mengkrog Holen
1Centre for Integrative Genetics (CIGENE), Department of Animal and Aquacultural Sciences, Faculty of Biosciences, Norwegian University of Life Sciences, NO-1432 Ås, Norway
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Simen Rød Sandve
1Centre for Integrative Genetics (CIGENE), Department of Animal and Aquacultural Sciences, Faculty of Biosciences, Norwegian University of Life Sciences, NO-1432 Ås, Norway
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Thomas Nelson Harvey
1Centre for Integrative Genetics (CIGENE), Department of Animal and Aquacultural Sciences, Faculty of Biosciences, Norwegian University of Life Sciences, NO-1432 Ås, Norway
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Yang Jin
1Centre for Integrative Genetics (CIGENE), Department of Animal and Aquacultural Sciences, Faculty of Biosciences, Norwegian University of Life Sciences, NO-1432 Ås, Norway
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Inga Leena Angell
2Department of Chemistry, Biotechnology and Food Science (IKBM), Norwegian University of Life Sciences, NO-1432 Ås, Norway
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Knut Rudi
2Department of Chemistry, Biotechnology and Food Science (IKBM), Norwegian University of Life Sciences, NO-1432 Ås, Norway
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Matthew Peter Kent
1Centre for Integrative Genetics (CIGENE), Department of Animal and Aquacultural Sciences, Faculty of Biosciences, Norwegian University of Life Sciences, NO-1432 Ås, Norway
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  • For correspondence: matthew.peter.kent@nmbu.no
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ABSTRACT

Background Chitin is a common component in the natural diet of many fish, and a range of chitinases with the potential to down chitin have been identified. Yet whether chitin is metabolized in fish is still unclear. Here we used a combination of chitinase activity assay, transcriptomics, and 16S rRNA bacterial analysis to assess the effect of chitin supplementation on Atlantic salmon gene expression and microbial community.

Results Atlantic salmon express multiple genes associated with chitin metabolism, and we show that the expression and activity of Atlantic salmon chitinases are not affected by the addition of dietary chitin. We do, however, demonstrate an association between gut microbial composition, chitinase activity in the gut, and host chitinase expression.

Conclusion The findings presented here support the idea that chitin metabolism genes are linked to the maintenance of a chitin-based barrier in the teleost gut. These results contribute to a greater understanding of chitin metabolism in fish.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-10594/

  • https://www.ncbi.nlm.nih.gov/bioproject/PRJNA820557/

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 05, 2022.
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The effect of dietary chitin on Atlantic salmon (Salmo salar) chitinase activity, gene expression, and microbial composition
Matilde Mengkrog Holen, Simen Rød Sandve, Thomas Nelson Harvey, Yang Jin, Inga Leena Angell, Knut Rudi, Matthew Peter Kent
bioRxiv 2022.05.05.490722; doi: https://doi.org/10.1101/2022.05.05.490722
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The effect of dietary chitin on Atlantic salmon (Salmo salar) chitinase activity, gene expression, and microbial composition
Matilde Mengkrog Holen, Simen Rød Sandve, Thomas Nelson Harvey, Yang Jin, Inga Leena Angell, Knut Rudi, Matthew Peter Kent
bioRxiv 2022.05.05.490722; doi: https://doi.org/10.1101/2022.05.05.490722

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