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RanBP1 plays an essential role in directed migration of neural crest cells during development

View ORCID ProfileElias H Barriga, Delan N Alasaadi, Chiara Mencarelli, View ORCID ProfileRoberto Mayor, View ORCID ProfileFranck Pichaud
doi: https://doi.org/10.1101/2022.05.05.490747
Elias H Barriga
1Cell and developmental Biology, University College London, London, WC1E 6BT, United Kingdom
2Mechanisms of Morphogenesis Lab, Instituto Gulbenkian de Ciencia, Oeiras, 2780-156, Portugal
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  • ORCID record for Elias H Barriga
Delan N Alasaadi
1Cell and developmental Biology, University College London, London, WC1E 6BT, United Kingdom
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Chiara Mencarelli
3Laboratory for Molecular Cell Biology, University College London, London, WC1E 6BT, United Kingdom
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Roberto Mayor
1Cell and developmental Biology, University College London, London, WC1E 6BT, United Kingdom
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  • For correspondence: r.mayor@ucl.ac.uk f.pichaud@ucl.ac.uk
Franck Pichaud
3Laboratory for Molecular Cell Biology, University College London, London, WC1E 6BT, United Kingdom
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  • For correspondence: r.mayor@ucl.ac.uk f.pichaud@ucl.ac.uk
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ABSTRACT

Collective cell migration is essential for embryonic development, tissue regeneration and repair, and has been implicated in pathological conditions such as cancer metastasis. It is, in part, directed by external cues that promote front-to-rear polarity in individual cells. However, our understanding of the pathways that underpin the directional movement of cells in response to external cues remains incomplete. To examine this issue we made use of neural crest cells (NC), which migrate as a collective during development to generate vital structures including bones and cartilage. Using a candidate approach, we found an essential role for Ran-binding protein 1 (RanBP1), a key effector of the nucleocytoplasmic transport pathway, in enabling directed migration of these cells. Our results indicate that RanBP1 is required for establishing front-to-rear polarity, so that NCs are able to chemotax. Moreover, our work suggests that RanBP1 function in chemotaxis involves the polarity kinase LKB1/PAR4. We envisage that regulated nuclear export of LKB1 through Ran/RanBP1 is a key regulatory step required for establishing front-to-rear polarity and thus chemotaxis, during NC collective migration.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • ↵# Co-first authorship

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted May 07, 2022.
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RanBP1 plays an essential role in directed migration of neural crest cells during development
Elias H Barriga, Delan N Alasaadi, Chiara Mencarelli, Roberto Mayor, Franck Pichaud
bioRxiv 2022.05.05.490747; doi: https://doi.org/10.1101/2022.05.05.490747
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RanBP1 plays an essential role in directed migration of neural crest cells during development
Elias H Barriga, Delan N Alasaadi, Chiara Mencarelli, Roberto Mayor, Franck Pichaud
bioRxiv 2022.05.05.490747; doi: https://doi.org/10.1101/2022.05.05.490747

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