Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Developmental dynamics of the postsynaptic proteome to understand synaptic maturation and dysmaturation

Takeshi Kaizuka, Takehiro Suzuki, Noriyuki Kishi, Manfred W. Kilimann, Takehiko Ueyama, Masahiko Watanabe, Hideyuki Okano, Naoshi Dohmae, View ORCID ProfileToru Takumi
doi: https://doi.org/10.1101/2022.05.05.490828
Takeshi Kaizuka
1RIKEN Brain Science Institute, Wako, Saitama 351-0198, Japan
2Department Physiology and Cell Biology, Kobe University School of Medicine, Chuo, Kobe 650-0117, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Takehiro Suzuki
3Biomolecular Characterization Unit, RIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Noriyuki Kishi
1RIKEN Brain Science Institute, Wako, Saitama 351-0198, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Manfred W. Kilimann
4Max Planck Institute for Experimental Medicine, Göttingen, 37075, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Takehiko Ueyama
5Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Nada, Kobe 657-8501, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Masahiko Watanabe
6Department of Anatomy, Faculty of Medicine, Hokkaido University, Kita, Sapporo 060-8638, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hideyuki Okano
1RIKEN Brain Science Institute, Wako, Saitama 351-0198, Japan
7Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo 160-8585, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Naoshi Dohmae
3Biomolecular Characterization Unit, RIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Toru Takumi
1RIKEN Brain Science Institute, Wako, Saitama 351-0198, Japan
2Department Physiology and Cell Biology, Kobe University School of Medicine, Chuo, Kobe 650-0117, Japan
8RIKEN Center for Biosystems Dynamics Research, Chuo, Kobe 650-0047, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Toru Takumi
  • For correspondence: takumit@med.kobe-u.ac.jp
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

The postsynaptic density (PSD) is a protein condensate composed of ∼1,000 proteins beneath the postsynaptic membrane of excitatory synapses. The number, shape, and plasticity of synapses are altered during development. However, the dynamics of synaptic protein composition across development have not been fully understood. Here we show alterations of PSD protein composition in mouse and primate brains during development. Proteins involved in synapse regulation are enriched in the differentially expressed (288 decreased and 267 increased) proteins on mouse PSD after a 2-week-old. We find that the changes in PSD protein abundance in mouse brains correlate with gene expression levels in postnatal mice and perinatal primates. This alteration of PSD composition is likely to be defective in the brains of mouse models or patients with autism spectrum disorder (ASD). Finally, we demonstrate that the brain of the common marmoset (Callithrix jacchus) changes PSD composition after the juvenile period. The alteration of PSD composition after 2-month-old is distinct from that observed in mice. Our results provide a comprehensive architecture of the remodeling of PSD composition across development, which may explain the molecular basics of synapse maturation and the pathology of psychiatric disorders, such as ASD.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • ↵9 Lead Contact

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted May 06, 2022.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Developmental dynamics of the postsynaptic proteome to understand synaptic maturation and dysmaturation
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Developmental dynamics of the postsynaptic proteome to understand synaptic maturation and dysmaturation
Takeshi Kaizuka, Takehiro Suzuki, Noriyuki Kishi, Manfred W. Kilimann, Takehiko Ueyama, Masahiko Watanabe, Hideyuki Okano, Naoshi Dohmae, Toru Takumi
bioRxiv 2022.05.05.490828; doi: https://doi.org/10.1101/2022.05.05.490828
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Developmental dynamics of the postsynaptic proteome to understand synaptic maturation and dysmaturation
Takeshi Kaizuka, Takehiro Suzuki, Noriyuki Kishi, Manfred W. Kilimann, Takehiko Ueyama, Masahiko Watanabe, Hideyuki Okano, Naoshi Dohmae, Toru Takumi
bioRxiv 2022.05.05.490828; doi: https://doi.org/10.1101/2022.05.05.490828

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (3689)
  • Biochemistry (7797)
  • Bioengineering (5676)
  • Bioinformatics (21290)
  • Biophysics (10578)
  • Cancer Biology (8176)
  • Cell Biology (11945)
  • Clinical Trials (138)
  • Developmental Biology (6763)
  • Ecology (10401)
  • Epidemiology (2065)
  • Evolutionary Biology (13867)
  • Genetics (9708)
  • Genomics (13073)
  • Immunology (8146)
  • Microbiology (20014)
  • Molecular Biology (7853)
  • Neuroscience (43058)
  • Paleontology (320)
  • Pathology (1279)
  • Pharmacology and Toxicology (2258)
  • Physiology (3353)
  • Plant Biology (7232)
  • Scientific Communication and Education (1312)
  • Synthetic Biology (2006)
  • Systems Biology (5538)
  • Zoology (1128)