Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Single neurons and networks in the claustrum integrate input from widespread cortical sources

View ORCID ProfileAndrew M. Shelton, View ORCID ProfileDavid K. Oliver, Joachim S. Grimstvedt, Ivan P. Lazarte, Ishaan Kapoor, Jake A. Swann, View ORCID ProfileCaitlin A. Ashcroft, Simon N. Williams, View ORCID ProfileNiall Conway, View ORCID ProfileAmy Robinson, View ORCID ProfileClifford G. Kentros, View ORCID ProfileMenno P. Witter, View ORCID ProfileSimon J.B. Butt, View ORCID ProfileAdam M. Packer
doi: https://doi.org/10.1101/2022.05.06.490864
Andrew M. Shelton
1Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Andrew M. Shelton
  • For correspondence: adampacker@gmail.com AndrewMcDowallShelton@gmail.com
David K. Oliver
1Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for David K. Oliver
Joachim S. Grimstvedt
2Kavli Institute for Systems Neuroscience, Centre for Neural Computation, Norwegian University of Science and Technology, Trondheim, Norway
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ivan P. Lazarte
1Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ishaan Kapoor
1Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jake A. Swann
1Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Caitlin A. Ashcroft
1Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Caitlin A. Ashcroft
Simon N. Williams
1Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Niall Conway
1Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Niall Conway
Amy Robinson
2Kavli Institute for Systems Neuroscience, Centre for Neural Computation, Norwegian University of Science and Technology, Trondheim, Norway
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Amy Robinson
Clifford G. Kentros
2Kavli Institute for Systems Neuroscience, Centre for Neural Computation, Norwegian University of Science and Technology, Trondheim, Norway
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Clifford G. Kentros
Menno P. Witter
2Kavli Institute for Systems Neuroscience, Centre for Neural Computation, Norwegian University of Science and Technology, Trondheim, Norway
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Menno P. Witter
Simon J.B. Butt
1Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Simon J.B. Butt
Adam M. Packer
1Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Adam M. Packer
  • For correspondence: adampacker@gmail.com AndrewMcDowallShelton@gmail.com
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

The claustrum is highly interconnected with many structures in the brain, but the organizing principles governing its vast connectivity have yet to be fully explored. We investigated the defining characteristics and activity of single claustrum neurons, the nature of their relationship with the cortex, and their connectivity within the claustrum itself to gain a comprehensive view of claustral circuits. We show that the claustrum is composed of excitatory and inhibitory cell types that are connected through intraclaustral excitatory synapses, especially between neurons with disparate projection targets. Investigations of corticoclaustral innervation demonstrated that axons from the cortex localize to distinct dorsoventral modules depending on their region of origin. In vitro dual-color optogenetic mapping experiments revealed that individual claustrum neurons integrated inputs from more than one cortical region in a cell type- and projection target-specific manner. Integration in claustrum neurons was observed to be particularly common between areas of the frontal cortex and less so for sensory, motor, and association cortices. Finally, activity in claustrum axons in midline cortical areas recorded with in vivo two-photon calcium imaging showed responses to multimodal sensory stimuli. Our findings shed light on the organizing principles of claustrum circuits, demonstrating that individual claustrum neurons integrate cortical inputs and redistribute this information back to cortex after performing output target- and cell type-dependent local computations.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted May 06, 2022.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Single neurons and networks in the claustrum integrate input from widespread cortical sources
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Single neurons and networks in the claustrum integrate input from widespread cortical sources
Andrew M. Shelton, David K. Oliver, Joachim S. Grimstvedt, Ivan P. Lazarte, Ishaan Kapoor, Jake A. Swann, Caitlin A. Ashcroft, Simon N. Williams, Niall Conway, Amy Robinson, Clifford G. Kentros, Menno P. Witter, Simon J.B. Butt, Adam M. Packer
bioRxiv 2022.05.06.490864; doi: https://doi.org/10.1101/2022.05.06.490864
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Single neurons and networks in the claustrum integrate input from widespread cortical sources
Andrew M. Shelton, David K. Oliver, Joachim S. Grimstvedt, Ivan P. Lazarte, Ishaan Kapoor, Jake A. Swann, Caitlin A. Ashcroft, Simon N. Williams, Niall Conway, Amy Robinson, Clifford G. Kentros, Menno P. Witter, Simon J.B. Butt, Adam M. Packer
bioRxiv 2022.05.06.490864; doi: https://doi.org/10.1101/2022.05.06.490864

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (3601)
  • Biochemistry (7567)
  • Bioengineering (5521)
  • Bioinformatics (20782)
  • Biophysics (10325)
  • Cancer Biology (7978)
  • Cell Biology (11634)
  • Clinical Trials (138)
  • Developmental Biology (6602)
  • Ecology (10200)
  • Epidemiology (2065)
  • Evolutionary Biology (13610)
  • Genetics (9539)
  • Genomics (12844)
  • Immunology (7919)
  • Microbiology (19538)
  • Molecular Biology (7657)
  • Neuroscience (42080)
  • Paleontology (308)
  • Pathology (1257)
  • Pharmacology and Toxicology (2201)
  • Physiology (3267)
  • Plant Biology (7038)
  • Scientific Communication and Education (1294)
  • Synthetic Biology (1951)
  • Systems Biology (5426)
  • Zoology (1116)