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Allogeneic testes transplanted into partially castrated adult medaka (Oryzias latipes) can produce donor-derived offspring by natural mating over a prolonged period

Daichi Kayo, Shinji Kanda, Kataaki Okubo
doi: https://doi.org/10.1101/2022.05.07.490875
Daichi Kayo
1Department of Aquatic Bioscience, Graduate School of Agricultural and Life Science, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan
2Laboratory of Molecular Ethology, Department of Integrative Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980-8577, Japan
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  • For correspondence: daichi.kayo.b8@tohoku.ac.jp
Shinji Kanda
3Laboratory of Physiology, Atmosphere and Ocean Research Institute, The University of Tokyo, Kashiwa, Chiba 277-8564, Japan
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Kataaki Okubo
1Department of Aquatic Bioscience, Graduate School of Agricultural and Life Science, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan
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Abstract

Germ cell transplantation protocols generally require induced immunosuppression in the recipient to avoid rejection of the transplanted cells; cytotoxic drugs may also be used to deprive the recipient germ cells and create a germ cell niche that allows donor-derived germ cells to thrive. In the present study, we demonstrate that in medaka, allogeneic adult testicular tissue will engraft in adult recipients immediately after the rough castration of the recipients and without use of immunosuppressive drugs. The allografted testes are retained in the recipient body for at least three months and are able to produce viable gametes that yield offspring after natural mating. Some recipients showed a high frequency (over 60%) of offspring derived from spermatozoa produced by the transplanted germ cell tissues. Histological analyses showed that allografted testicular tissues included both germ cells and somatic cells that had established within an immunocompetent recipient testis. The relative simplicity of this new approach will benefit investigations of the basic processes of reproductive immunology and will improve the technique of gonadal tissue transplantation.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted June 16, 2022.
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Allogeneic testes transplanted into partially castrated adult medaka (Oryzias latipes) can produce donor-derived offspring by natural mating over a prolonged period
Daichi Kayo, Shinji Kanda, Kataaki Okubo
bioRxiv 2022.05.07.490875; doi: https://doi.org/10.1101/2022.05.07.490875
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Allogeneic testes transplanted into partially castrated adult medaka (Oryzias latipes) can produce donor-derived offspring by natural mating over a prolonged period
Daichi Kayo, Shinji Kanda, Kataaki Okubo
bioRxiv 2022.05.07.490875; doi: https://doi.org/10.1101/2022.05.07.490875

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