Article Information
- May 11, 2022.
Author Information
- Carlos A. M. Cardoso-Junior1,7,
- Gustavo J. Tibério1,7,
- Denyse C. Lago2,
- Luiz Carlos Vieira1,
- José C. Rosa1,
- Alexandre R. Paschoal3,
- Isobel Ronai4,
- Benjamin P. Oldroyd4,5 and
- Klaus Hartfelder1,2,6,*
- 1Departamento de Biologia Celular e Molecular, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
- 2Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
- 3Universidade Tecnológica Federal do Paraná, Cornélio Procópio, PR, Brazil.
- 4Behaviour, Ecology and Evolution (BEE) laboratory, Macleay Building A12, University of Sydney, Sydney NSW 2006, Australia.
- 5Wissenschaftskolleg zu Berlin, Wallotstrasse 19 D-14193, Berlin, Germany.
- ↵* Correspondence: klaus{at}fmrp.usp.br.
↵7 These authors contributed equally.
AUTHOR CONTRIBUTIONS CAM designed, conceived, performed and analyzed the experiments on adult bees, helped in the pulldown assay, cloned and sequenced the full-length lncov1 sequence, designed the RNA-seq analyses together with LCV and wrote the manuscript draft together with KH. GJT conceived, designed the study, performed and analyzed the larval experiments. DCL performed the JH treatment experiment, analyzed FISH assays together with GJT and quantified the caste-specific Tudor-SN gene expression. LCV performed the bioinformatic analyses for RNA-seq datasets. JCR and GJT performed the mass spectrometry analysis. IR helped in gene expression analyses of adult honey bees and in the setting up of the cages for the diet and QMP treatments. BPO designed the experiments with adult bees together with CAM, performed social manipulations in field colonies, and supervised the study. ARP performed the bioinformatics analysis on the conservation of the lncov1 gene. KH conceived, designed, supervised the study, and, together with CAM drafted the manuscript. All authors contributed critically to this paper and approved the final version.