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Extending of imaging volume in soft x-ray tomography

Axel Ekman, View ORCID ProfileJian-Hua Chen, View ORCID ProfileBieke Vanslembrouck, Carolyn A Larabell, Mark A Le Gros, View ORCID ProfileVenera Weinhardt
doi: https://doi.org/10.1101/2022.05.11.491437
Axel Ekman
1University of California San Francisco, Department of Anatomy, San Francisco, 94143 California, United States of America
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Jian-Hua Chen
1University of California San Francisco, Department of Anatomy, San Francisco, 94143 California, United States of America
2Lawrence Berkeley National Laboratory, Molecular Biophysics and Integrated Bioimaging Division, Berkeley, 94720 California, United States of America
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Bieke Vanslembrouck
1University of California San Francisco, Department of Anatomy, San Francisco, 94143 California, United States of America
2Lawrence Berkeley National Laboratory, Molecular Biophysics and Integrated Bioimaging Division, Berkeley, 94720 California, United States of America
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Carolyn A Larabell
1University of California San Francisco, Department of Anatomy, San Francisco, 94143 California, United States of America
2Lawrence Berkeley National Laboratory, Molecular Biophysics and Integrated Bioimaging Division, Berkeley, 94720 California, United States of America
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Mark A Le Gros
1University of California San Francisco, Department of Anatomy, San Francisco, 94143 California, United States of America
2Lawrence Berkeley National Laboratory, Molecular Biophysics and Integrated Bioimaging Division, Berkeley, 94720 California, United States of America
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Venera Weinhardt
1University of California San Francisco, Department of Anatomy, San Francisco, 94143 California, United States of America
3Heidelberg University, Centre for Organismal Studies, 69120 Heidelberg, Germany
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  • For correspondence: venera.weinhardt@cos.uni-heidelberg.de
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Abstract

Soft x-ray tomography offers rapid whole single cell imaging with a few tens of nanometers spatial resolution without fixation or labelling. At the moment, this technique is limited to 10 μm thick specimens, such that applications of soft x-ray tomography to large human cells or multicellular specimens are not possible. We have developed a theoretical and experimental framework for soft x-ray tomography to enable extension of imaging volume to 18 μm thick specimens. This approach, based on long depth of field and half-acquisition tomography, is easily applicable to existing full-rotation based microscopes. This opens applications for imaging of large human cells, which are often observed in cancer research and cell to cell interactions.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 11, 2022.
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Extending of imaging volume in soft x-ray tomography
Axel Ekman, Jian-Hua Chen, Bieke Vanslembrouck, Carolyn A Larabell, Mark A Le Gros, Venera Weinhardt
bioRxiv 2022.05.11.491437; doi: https://doi.org/10.1101/2022.05.11.491437
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Extending of imaging volume in soft x-ray tomography
Axel Ekman, Jian-Hua Chen, Bieke Vanslembrouck, Carolyn A Larabell, Mark A Le Gros, Venera Weinhardt
bioRxiv 2022.05.11.491437; doi: https://doi.org/10.1101/2022.05.11.491437

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