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DNA double strand breaks lead to de novo transcription and translation of damage-induced long RNAs in planta

Tom Schreiber, Sunita Tripathee, Thomas Iwen, Anja Prange, Khabat Vahabi, Ramona Grützner, Claudia Horn, Sylvestre Marillonnet, View ORCID ProfileAlain Tissier
doi: https://doi.org/10.1101/2022.05.11.491484
Tom Schreiber
Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), Germany
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  • For correspondence: tom.schreiber@ipb-halle.de alain.tissier@ipb-halle.de
Sunita Tripathee
Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), Germany
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Thomas Iwen
Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), Germany
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Anja Prange
Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), Germany
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Khabat Vahabi
Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), Germany
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Ramona Grützner
Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), Germany
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Claudia Horn
Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), Germany
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Sylvestre Marillonnet
Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), Germany
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Alain Tissier
Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), Germany
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  • ORCID record for Alain Tissier
  • For correspondence: tom.schreiber@ipb-halle.de alain.tissier@ipb-halle.de
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ABSTRACT

DNA double strand breaks (DSBs) are lethal threats that need to be repaired. Although many of the proteins involved in the early steps of DSB repair have been characterized, recent reports indicate that damage induced long and small RNAs also play an important role in DSB repair. Here, using a Nicotiana benthamiana transgenic line originally designed as a reporter for targeted knock-ins, we show that DSBs generated by Cas9 induce the transcription of long stable RNAs (damage-induced long RNAs - dilRNAs) that are translated into proteins. Using an array of single guide RNAs we show that the initiation of transcription takes place in the vicinity of the DSB. Single strand DNA nicks are not able to induce transcription, showing that cis DNA damage-induced transcription is specific for DSBs. Our results support a model in which a default and early event in the processing of DSBs is transcription into RNA which, depending on the genomic and genic context, can undergo distinct fates, including translation into protein, degradation or production of small RNAs. Our results have general implications for understanding the role of transcription in the repair of DSBs and, reciprocally, reveal DSBs as yet another way to regulate gene expression.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted May 11, 2022.
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DNA double strand breaks lead to de novo transcription and translation of damage-induced long RNAs in planta
Tom Schreiber, Sunita Tripathee, Thomas Iwen, Anja Prange, Khabat Vahabi, Ramona Grützner, Claudia Horn, Sylvestre Marillonnet, Alain Tissier
bioRxiv 2022.05.11.491484; doi: https://doi.org/10.1101/2022.05.11.491484
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DNA double strand breaks lead to de novo transcription and translation of damage-induced long RNAs in planta
Tom Schreiber, Sunita Tripathee, Thomas Iwen, Anja Prange, Khabat Vahabi, Ramona Grützner, Claudia Horn, Sylvestre Marillonnet, Alain Tissier
bioRxiv 2022.05.11.491484; doi: https://doi.org/10.1101/2022.05.11.491484

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