Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Phosphorylation Status of B Beta Subunit Controls PP2A activity in Ethylene-mediated Root Growth Inhibition

Zhengyao Shao, Bo Zhao, Prashanth Kotla, Jackson G. Burns, Jaclyn Tran, Meiyu Ke, Xu Chen, Karen S. Browning, Hong Qiao
doi: https://doi.org/10.1101/2022.05.11.491551
Zhengyao Shao
1Institute for Cellular and Molecular Biology and The University of Texas at Austin, Austin, Texas 78712, USA
2Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bo Zhao
1Institute for Cellular and Molecular Biology and The University of Texas at Austin, Austin, Texas 78712, USA
2Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Prashanth Kotla
1Institute for Cellular and Molecular Biology and The University of Texas at Austin, Austin, Texas 78712, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jackson G. Burns
1Institute for Cellular and Molecular Biology and The University of Texas at Austin, Austin, Texas 78712, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jaclyn Tran
1Institute for Cellular and Molecular Biology and The University of Texas at Austin, Austin, Texas 78712, USA
2Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Meiyu Ke
3Haixia Institute of Science and Technology, Horticultural Plant Biology and Metabolomics Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xu Chen
3Haixia Institute of Science and Technology, Horticultural Plant Biology and Metabolomics Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Karen S. Browning
1Institute for Cellular and Molecular Biology and The University of Texas at Austin, Austin, Texas 78712, USA
2Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hong Qiao
1Institute for Cellular and Molecular Biology and The University of Texas at Austin, Austin, Texas 78712, USA
2Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: hqiao@austin.utexas.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

The various combinations and regulations of different subunits of phosphatase PP2A holoenzymes underlie their functional complexity and importance. We identified a distinct PP2A holoenzyme composed of A2, Bβ and C4. Upon the ethylene treatment, Bβ subunit is dephosphorylated, leading to a tight formation of the A2-C4-Bβ protein complex. EIR1 is the target of the PP2A A2-C4-Bβ complex mediated by the A2 subunit. Importantly, the phosphorylation of Bβ subunit in the absence of ethylene leads to an inactivation of PP2A. As a result, EIR1 remains phosphorylated, and no root growth inhibition occurs. Upon the ethylene treatment, Bβ subunit is dephosphorylated, leading to an activation of PP2A to dephosphorylate EIR1, resulting in a root growth inhibition. Altogether, our research revealed a novel molecular mechanism by which the dephosphorylation of Bβ subunit switches on the PP2A activity to dephosphorylate EIR1, leading to a root growth inhibition in response to ethylene.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted May 11, 2022.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Phosphorylation Status of B Beta Subunit Controls PP2A activity in Ethylene-mediated Root Growth Inhibition
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Phosphorylation Status of B Beta Subunit Controls PP2A activity in Ethylene-mediated Root Growth Inhibition
Zhengyao Shao, Bo Zhao, Prashanth Kotla, Jackson G. Burns, Jaclyn Tran, Meiyu Ke, Xu Chen, Karen S. Browning, Hong Qiao
bioRxiv 2022.05.11.491551; doi: https://doi.org/10.1101/2022.05.11.491551
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Phosphorylation Status of B Beta Subunit Controls PP2A activity in Ethylene-mediated Root Growth Inhibition
Zhengyao Shao, Bo Zhao, Prashanth Kotla, Jackson G. Burns, Jaclyn Tran, Meiyu Ke, Xu Chen, Karen S. Browning, Hong Qiao
bioRxiv 2022.05.11.491551; doi: https://doi.org/10.1101/2022.05.11.491551

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Plant Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3514)
  • Biochemistry (7365)
  • Bioengineering (5342)
  • Bioinformatics (20318)
  • Biophysics (10041)
  • Cancer Biology (7773)
  • Cell Biology (11348)
  • Clinical Trials (138)
  • Developmental Biology (6450)
  • Ecology (9979)
  • Epidemiology (2065)
  • Evolutionary Biology (13354)
  • Genetics (9370)
  • Genomics (12607)
  • Immunology (7724)
  • Microbiology (19087)
  • Molecular Biology (7459)
  • Neuroscience (41134)
  • Paleontology (300)
  • Pathology (1235)
  • Pharmacology and Toxicology (2142)
  • Physiology (3177)
  • Plant Biology (6878)
  • Scientific Communication and Education (1276)
  • Synthetic Biology (1900)
  • Systems Biology (5328)
  • Zoology (1091)