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Assessment of 3D MINFLUX data for quantitative structural biology in cells revisited

View ORCID ProfileKlaus C. Gwosch, View ORCID ProfileFrancisco Balzarotti, View ORCID ProfileJasmin K. Pape, View ORCID ProfilePhilipp Hoess, View ORCID ProfileJan Ellenberg, View ORCID ProfileJonas Ries, View ORCID ProfileUlf Matti, View ORCID ProfileRoman Schmidt, View ORCID ProfileSteffen J. Sahl, View ORCID ProfileStefan W. Hell
doi: https://doi.org/10.1101/2022.05.13.491065
Klaus C. Gwosch
1Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany
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Francisco Balzarotti
1Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany
2Research Institute of Molecular Pathology, Vienna, Austria
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Jasmin K. Pape
1Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany
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Philipp Hoess
3Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
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Jan Ellenberg
3Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
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Jonas Ries
3Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
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Ulf Matti
4Abberior Instruments GmbH, Göttingen, Germany
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Roman Schmidt
4Abberior Instruments GmbH, Göttingen, Germany
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Steffen J. Sahl
1Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany
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Stefan W. Hell
1Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany
5Department of Optical Nanoscopy, Max Planck Institute for Medical Research, Heidelberg, Germany
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  • For correspondence: shell@gwdg.de
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Abstract

Prakash and Curd provide a re-analysis1 of individual datasets taken from our report2 demonstrating MINFLUX 3D imaging in cells. Their evaluation confirms the unique localization precision provided by MINFLUX2,3 featuring a standard deviation of σ = 1-3 nm. We appreciate their confirmation and also welcome the opportunity to clarify their remaining points. The hitherto almost unconceivable 3D localization precision attained by MINFLUX is likely to hold the key to an all-optical dynamical structural biology.

Competing Interest Statement

S.W.H. is a co-founder of the company Abberior Instruments, which commercializes super-resolution microscopy systems, including MINFLUX. S.W.H., K.C.G. and F.B. hold patents on the principles, embodiments and procedures of MINFLUX.

Footnotes

  • ↵* Email: stefan.hell{at}mpinat.mpg.de.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted May 17, 2022.
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Assessment of 3D MINFLUX data for quantitative structural biology in cells revisited
Klaus C. Gwosch, Francisco Balzarotti, Jasmin K. Pape, Philipp Hoess, Jan Ellenberg, Jonas Ries, Ulf Matti, Roman Schmidt, Steffen J. Sahl, Stefan W. Hell
bioRxiv 2022.05.13.491065; doi: https://doi.org/10.1101/2022.05.13.491065
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Assessment of 3D MINFLUX data for quantitative structural biology in cells revisited
Klaus C. Gwosch, Francisco Balzarotti, Jasmin K. Pape, Philipp Hoess, Jan Ellenberg, Jonas Ries, Ulf Matti, Roman Schmidt, Steffen J. Sahl, Stefan W. Hell
bioRxiv 2022.05.13.491065; doi: https://doi.org/10.1101/2022.05.13.491065

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