Assessment of 3D MINFLUX data for quantitative structural biology in cells revisited

Klaus C. Gwosch; Francisco Balzarotti; Jasmin K. Pape; Philipp Hoess; Jan Ellenberg; Jonas Ries; Ulf Matti; Roman Schmidt; Steffen J. Sahl; Stefan W. Hell

doi:10.1101/2022.05.13.491065

Abstract

Prakash and Curd provide a re-analysis¹ of individual datasets taken from our report² demonstrating MINFLUX 3D imaging in cells. Their evaluation confirms the unique localization precision provided by MINFLUX^2,3 featuring a standard deviation of σ = 1-3 nm. We appreciate their confirmation and also welcome the opportunity to clarify their remaining points. The hitherto almost unconceivable 3D localization precision attained by MINFLUX is likely to hold the key to an all-optical dynamical structural biology.

Competing Interest Statement

S.W.H. is a co-founder of the company Abberior Instruments, which commercializes super-resolution microscopy systems, including MINFLUX. S.W.H., K.C.G. and F.B. hold patents on the principles, embodiments and procedures of MINFLUX.

Footnotes

↵* Email: stefan.hell{at}mpinat.mpg.de.

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