Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Multiplexed chromatin imaging reveals predominantly pairwise long-range coordination between Drosophila Polycomb genes

Julian Gurgo, Jean-Charles Walter, Jean-Bernard Fiche, Christophe Houbron, Marie Schaeffer, View ORCID ProfileGiacomo Cavalli, Frédéric Bantignies, View ORCID ProfileMarcelo Nollmann
doi: https://doi.org/10.1101/2022.05.16.492046
Julian Gurgo
1Centre de Biochimie Structurale, CNRS UMR 5048, INSERM U1054, Université de Montpellier, 60 rue de Navacelles, 34090, Montpellier, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jean-Charles Walter
3Laboratoire Charles Coulomb (L2C), Univ. Montpellier, CNRS, Montpellier, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jean-Bernard Fiche
1Centre de Biochimie Structurale, CNRS UMR 5048, INSERM U1054, Université de Montpellier, 60 rue de Navacelles, 34090, Montpellier, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christophe Houbron
1Centre de Biochimie Structurale, CNRS UMR 5048, INSERM U1054, Université de Montpellier, 60 rue de Navacelles, 34090, Montpellier, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marie Schaeffer
1Centre de Biochimie Structurale, CNRS UMR 5048, INSERM U1054, Université de Montpellier, 60 rue de Navacelles, 34090, Montpellier, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Giacomo Cavalli
2Institut de Génétique Humaine, CNRS UMR 9002, Université de Montpellier, 141 rue de la Cardonille, 34396, Montpellier, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Giacomo Cavalli
Frédéric Bantignies
2Institut de Génétique Humaine, CNRS UMR 9002, Université de Montpellier, 141 rue de la Cardonille, 34396, Montpellier, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: frederic.bantignies@igh.cnrs.fr marcelo.nollmann@cbs.cnrs.fr
Marcelo Nollmann
1Centre de Biochimie Structurale, CNRS UMR 5048, INSERM U1054, Université de Montpellier, 60 rue de Navacelles, 34090, Montpellier, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Marcelo Nollmann
  • For correspondence: frederic.bantignies@igh.cnrs.fr marcelo.nollmann@cbs.cnrs.fr
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Polycomb (Pc) group proteins are transcriptional regulators with key roles in development, cell identity and differentiation. Pc-bound chromatin regions form repressive domains that interact in 3D to assemble repressive nuclear compartments. Here, we used multiplexed chromatin imaging to investigate whether Pc compartments involve the clustering of multiple Pc domains during Drosophila development. Notably, 3D proximity between Pc targets is rare and involves predominantly pairwise interactions. These 3D proximities are particularly enhanced in segments where Pc genes are co-repressed. In addition, segment-specific expression of Hox Pc targets leads to their spatial segregation from Pc repressed genes. Finally, non-Hox Pc targets are proximal in regions where they are co-expressed. These results indicate that long-range Pc interactions are temporally and spatially regulated during differentiation and development but do not involve clustering of multiple distant Pc genes.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted May 16, 2022.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Multiplexed chromatin imaging reveals predominantly pairwise long-range coordination between Drosophila Polycomb genes
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Multiplexed chromatin imaging reveals predominantly pairwise long-range coordination between Drosophila Polycomb genes
Julian Gurgo, Jean-Charles Walter, Jean-Bernard Fiche, Christophe Houbron, Marie Schaeffer, Giacomo Cavalli, Frédéric Bantignies, Marcelo Nollmann
bioRxiv 2022.05.16.492046; doi: https://doi.org/10.1101/2022.05.16.492046
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Multiplexed chromatin imaging reveals predominantly pairwise long-range coordination between Drosophila Polycomb genes
Julian Gurgo, Jean-Charles Walter, Jean-Bernard Fiche, Christophe Houbron, Marie Schaeffer, Giacomo Cavalli, Frédéric Bantignies, Marcelo Nollmann
bioRxiv 2022.05.16.492046; doi: https://doi.org/10.1101/2022.05.16.492046

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genetics
Subject Areas
All Articles
  • Animal Behavior and Cognition (4688)
  • Biochemistry (10379)
  • Bioengineering (7695)
  • Bioinformatics (26371)
  • Biophysics (13547)
  • Cancer Biology (10719)
  • Cell Biology (15460)
  • Clinical Trials (138)
  • Developmental Biology (8509)
  • Ecology (12842)
  • Epidemiology (2067)
  • Evolutionary Biology (16885)
  • Genetics (11415)
  • Genomics (15493)
  • Immunology (10638)
  • Microbiology (25254)
  • Molecular Biology (10239)
  • Neuroscience (54586)
  • Paleontology (402)
  • Pathology (1671)
  • Pharmacology and Toxicology (2899)
  • Physiology (4354)
  • Plant Biology (9263)
  • Scientific Communication and Education (1588)
  • Synthetic Biology (2561)
  • Systems Biology (6789)
  • Zoology (1470)