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A Murine Model of Abductor Insufficiency Accelerates the Development of Hip Osteoarthritis

Michael B. Geary, Caitlin A. Orner, Helen Shammas, John M. Reuter, Alayna E. Loiselle, View ORCID ProfileChia-Lung Wu, Brian D. Giordano
doi: https://doi.org/10.1101/2022.05.22.492940
Michael B. Geary
1Center for Musculoskeletal Research, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, NY 14642, USA
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Caitlin A. Orner
1Center for Musculoskeletal Research, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, NY 14642, USA
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Helen Shammas
1Center for Musculoskeletal Research, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, NY 14642, USA
3Department of Biomedical Engineering, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, NY 14642, USA
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John M. Reuter
1Center for Musculoskeletal Research, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, NY 14642, USA
2Department of Orthopaedics and Rehabilitation, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, NY 14642, USA
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Alayna E. Loiselle
1Center for Musculoskeletal Research, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, NY 14642, USA
2Department of Orthopaedics and Rehabilitation, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, NY 14642, USA
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Chia-Lung Wu
1Center for Musculoskeletal Research, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, NY 14642, USA
2Department of Orthopaedics and Rehabilitation, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, NY 14642, USA
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  • ORCID record for Chia-Lung Wu
  • For correspondence: chia-lung_wu@urmc.rochester.edu
Brian D. Giordano
1Center for Musculoskeletal Research, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, NY 14642, USA
2Department of Orthopaedics and Rehabilitation, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, NY 14642, USA
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Abstract

Osteoarthritis (OA) of the hip is a common and debilitating painful joint disease. A growing body of evidence suggests that there may be an association between periarticular myotendinous pathology and the development of hip OA. Thus, we hypothesized that a murine model of hip OA could be achieved through selective injury of the abductor complex around the hip. C57BL6/J mice were randomized to sham surgery or abductor injury, in which the myotendinous insertion at the third trochanter and greater trochanter were surgically detached. Mice were allowed free, active movement until sacrifice at either 3 weeks or 20 weeks post-injury. Histologic analyses and immunohistochemical staining (IHC) of the femoral head articular cartilage were performed, along with μCT analysis to assess subchondral bone remodeling. We observed that mice receiving abductor injury exhibited significant OA severity with loss of Type II Collagen staining compared to sham control mice at 20 weeks post-surgery, comparable MMPI13 expression was observed between injury and sham groups. No significant differences in subchondral bone were found on μCT after 20 weeks following injury. Our study suggests a link between abductor dysfunction and the development of hip OA, which are common pathomorphologies encountered in routine orthopaedic clinical practice. Further, this novel animal model may provide a valuable tool for future investigations into the pathogenesis and treatment of hip OA.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • ↵* co-first authors

  • ↵# co-last authors

  • Discussion is further extended.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted September 25, 2022.
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A Murine Model of Abductor Insufficiency Accelerates the Development of Hip Osteoarthritis
Michael B. Geary, Caitlin A. Orner, Helen Shammas, John M. Reuter, Alayna E. Loiselle, Chia-Lung Wu, Brian D. Giordano
bioRxiv 2022.05.22.492940; doi: https://doi.org/10.1101/2022.05.22.492940
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A Murine Model of Abductor Insufficiency Accelerates the Development of Hip Osteoarthritis
Michael B. Geary, Caitlin A. Orner, Helen Shammas, John M. Reuter, Alayna E. Loiselle, Chia-Lung Wu, Brian D. Giordano
bioRxiv 2022.05.22.492940; doi: https://doi.org/10.1101/2022.05.22.492940

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