Abstract
The African turquoise killifish is a powerful vertebrate system to study complex phenotypes at scale, including aging and age-related disease. Here we develop a rapid and precise CRISPR/Cas9-mediated knock-in approach in the killifish. We show its efficient application to precisely insert fluorescent reporters of different sizes at various genomic loci, to drive cell-type- and tissue-specific expression. This knock-in method should allow the establishment of humanized disease models and the development of cell-type-specific molecular probes for studying complex vertebrate biology.
Competing Interest Statement
The authors have declared no competing interest.
Abbreviations
- CN
- cortical nucleus
- CP
- central posterior thalamic nucleus
- Cpost
- posterior commissure
- DIL
- diffuse inferior lobe of hypothalamus
- gl
- glomerular layer
- Ha
- habenular nucleus
- Hc
- caudal hypothalamus
- Hd
- dorsal hypothalamus
- Hv
- ventral hypothalamus
- llf
- lateral longitudinal fascicle
- LR
- lateral recess of diencephalic ventricle
- mlf
- medial longitudinal fascicle
- MO
- medulla oblongata
- NG
- glomerular nucleus
- OB
- olfactory bulb
- ON
- optic nerve
- OT
- optic tectum
- PGZ
- periglomerular gray zone
- Tel
- telencephalon
- Tl
- torus longitudinalis
- TNa
- anterior tuberal nucleus
- TPp
- periventricular nucleus of posterior tuberculum
- Va
- valvula of cerebellum
- VAO
- ventral accessory optic nucleus
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Posted May 26, 2022.
