Iron regulates contrasting toxicity of uropathogenic Eschericia coli in macrophages and epithelial cells

Deepti Dabral; Hiren Ghosh; Masato Niwa; Tasuku Hirayama; Rinse de Boer; Marjon de Vos; Geert van den Bogaart

doi:10.1101/2022.05.29.493834

Delivery of iron-rich endosomes/lysosomes to XL1 containing vacuoles. Green: deoxygenated iron probe. Magenta: labelled XL1 blue.[supplements/493834_file03.mp4]
Delivery of iron-rich endosomes/lysosomes to XL1 containing vacuoles. Green: deoxygenated iron probe. Magenta: labelled XL1 blue.[supplements/493834_file04.mp4]
Cell 1 with overlay of BF channel is shown and unlabeled video.[supplements/493834_file05.mp4]
Delivery of iron-rich endosomes/lysosomes to ST999 containing vacuoles. Magenta: labelled bacteria. Green: deoxygenated iron probe.[supplements/493834_file06.mp4]
Delivery of iron-rich endosomes/lysosomes to ST999 containing vacuoles. Magenta: labelled bacteria. Green: deoxygenated iron probe.[supplements/493834_file07.mp4]
Delivery of iron-rich endosomes/lysosomes to ST999 containing vacuoles. Magenta: labelled bacteria. Green: deoxygenated iron probe.[supplements/493834_file08.mp4]
Delivery of iron-rich endosomes/lysosomes to ST999 containing vacuoles. Magenta: labelled bacteria. Green: deoxygenated iron probe.[supplements/493834_file09.mp4]
Cell 1 with overlay of BF channel is shown and unlabeled ST999.[supplements/493834_file10.mp4]
Fast movement of iron-rich endosomes/lysosomes, and macrophage swelling and blebbing indicative of its death by ST95. Magenta: ST95. Green: iron probe[supplements/493834_file11.mpg]
Fast movement of iron-rich endosomes/lysosomes, and macrophage swelling and blebbing indicative of its death by ST95. Magenta: ST95. Green: iron probe[supplements/493834_file12.avi]
Fast movement of iron-rich endosomes/lysosomes, and macrophage swelling and blebbing indicative of its death by ST95. Magenta: ST95. Green: iron probe[supplements/493834_file13.mpg]
Fast movement of iron-rich endosomes/lysosomes, and macrophage swelling and blebbing indicative of its death by ST95. Magenta: ST95. Green: iron probe[supplements/493834_file14.mpg]
Cell 1 with overlay of BF channel is shown and unlabeled ST95.[supplements/493834_file15.mp4]
Multiple ST999 within LAMP1 positive vacuoles.[supplements/493834_file16.mp4]