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Membrane wetting, molding and reticulation by protein condensates

View ORCID ProfileAgustín Mangiarotti, Nannan Chen, View ORCID ProfileZiliang Zhao, View ORCID ProfileReinhard Lipowsky, View ORCID ProfileRumiana Dimova
doi: https://doi.org/10.1101/2022.06.03.494704
Agustín Mangiarotti
1Max Planck Institute of Colloids and Interfaces, Science Park Golm, 14476 Potsdam, Germany
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  • ORCID record for Agustín Mangiarotti
Nannan Chen
1Max Planck Institute of Colloids and Interfaces, Science Park Golm, 14476 Potsdam, Germany
2Department of Nutrition and Food Hygiene, Guangzhou Medical University, Guangzhou 511436, China
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Ziliang Zhao
1Max Planck Institute of Colloids and Interfaces, Science Park Golm, 14476 Potsdam, Germany
3Leibniz Institute of Photonic Technology e.V., Albert-Einstein-Straße 9, 07745 Jena, Germany; and Institute of Applied Optics and Biophysics, Friedrich-Schiller-University Jena, Max-Wien Platz 1, 07743 Jena, Germany
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Reinhard Lipowsky
1Max Planck Institute of Colloids and Interfaces, Science Park Golm, 14476 Potsdam, Germany
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Rumiana Dimova
1Max Planck Institute of Colloids and Interfaces, Science Park Golm, 14476 Potsdam, Germany
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  • ORCID record for Rumiana Dimova
  • For correspondence: Rumiana.Dimova@mpikg.mpg.de
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Abstract

Cells compartmentalize their components in liquid-like condensates1, 2, 3, which can be reconstituted in vitro4, 5, 6. Although these condensates interact with membrane-bound organelles7, the potential of membrane remodeling and the underlying mechanisms are not well understood. Here, we demonstrate that interactions between protein condensates and membranes can lead to remarkable morphological transformations and describe these with theoretical analysis. Modulation of solution salinity or membrane composition drives the condensate-membrane system through two wetting transitions, from dewetting, through a broad regime of partial wetting, to complete wetting. The observed morphologies are governed by the interplay of adhesion, membrane elasticity and interfacial tension. A new phenomenon, namely reticulation or fingering of the condensate-membrane interface is observed when sufficient membrane area is available, producing complex curved structures. Our results highlight the relevance of wetting in cell biology, and pave the way for the design of synthetic membrane-droplet based biomaterials and compartments with tunable properties.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • The acknowledgements have been updated.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted June 28, 2022.
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Membrane wetting, molding and reticulation by protein condensates
Agustín Mangiarotti, Nannan Chen, Ziliang Zhao, Reinhard Lipowsky, Rumiana Dimova
bioRxiv 2022.06.03.494704; doi: https://doi.org/10.1101/2022.06.03.494704
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Membrane wetting, molding and reticulation by protein condensates
Agustín Mangiarotti, Nannan Chen, Ziliang Zhao, Reinhard Lipowsky, Rumiana Dimova
bioRxiv 2022.06.03.494704; doi: https://doi.org/10.1101/2022.06.03.494704

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