Abstract
Multiplexed cellular imaging typically relies on the sequential application of detection probes, such as antibodies or DNA barcodes, which is complex and time-consuming. To address this, we developed here protein nanobarcodes, composed of combinations of epitopes recognized by specific sets of nanobodies. The nanobarcodes are read in a single imaging step, relying on nanobodies conjugated to distinct fluorophores, which enables a precise analysis of large numbers of protein combinations.
Competing Interest Statement
S.O.R. and F.O. are shareholders of NanoTag Biotechnologies GmbH. All other authors declare no potential conflict of interest.
Footnotes
↵* danielle.dejong{at}med.uni-goettingen.de, mohsen.sadeghi{at}fu-berlin.de, srizzol{at}gwdg.de
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Posted June 04, 2022.
