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Optical Control of Neuronal Activities with Photoswitchable Nanovesicles

View ORCID ProfileHejian Xiong, Kevin A. Alberto, Jonghae Youn, Jaume Taura, Johannes Morstein, Xiuying Li, Yang Wang, Dirk Trauner, Paul A. Slesinger, Steven O. Nielsen, View ORCID ProfileZhenpeng Qin
doi: https://doi.org/10.1101/2022.06.10.495373
Hejian Xiong
1Department of Mechanical Engineering, The University of Texas at Dallas, Richardson, TX 75080, U.S.A.
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  • ORCID record for Hejian Xiong
Kevin A. Alberto
2Department of Chemistry, The University of Texas at Dallas, Richardson, TX 75080, U.S.A.
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Jonghae Youn
1Department of Mechanical Engineering, The University of Texas at Dallas, Richardson, TX 75080, U.S.A.
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Jaume Taura
3Nash Family Department of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, NY 10029, U.S.A.
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Johannes Morstein
4Department of Chemistry, New York University, New York, NY 10012, U.S.A.
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Xiuying Li
1Department of Mechanical Engineering, The University of Texas at Dallas, Richardson, TX 75080, U.S.A.
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Yang Wang
1Department of Mechanical Engineering, The University of Texas at Dallas, Richardson, TX 75080, U.S.A.
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Dirk Trauner
3Nash Family Department of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, NY 10029, U.S.A.
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Paul A. Slesinger
4Department of Chemistry, New York University, New York, NY 10012, U.S.A.
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  • For correspondence: Paul.Slesinger@mssm.edu Steven.Nielsen@utdallas.edu Zhenpeng.Qin@utdallas.edu
Steven O. Nielsen
2Department of Chemistry, The University of Texas at Dallas, Richardson, TX 75080, U.S.A.
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  • For correspondence: Paul.Slesinger@mssm.edu Steven.Nielsen@utdallas.edu Zhenpeng.Qin@utdallas.edu
Zhenpeng Qin
1Department of Mechanical Engineering, The University of Texas at Dallas, Richardson, TX 75080, U.S.A.
5Department of Bioengineering, The University of Texas at Dallas, Richardson, TX 75080, U.S.A.
6Department of Surgery, University of Texas at Southwestern Medical Center, Dallas, TX 75080, U.S.A.
7Center for Advanced Pain Studies, The University of Texas at Dallas, Richardson, TX 75080, U.S.A.
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  • For correspondence: Paul.Slesinger@mssm.edu Steven.Nielsen@utdallas.edu Zhenpeng.Qin@utdallas.edu
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ABSTRACT

Precise modulation of neuronal activity by neuroactive molecules is essential for understanding brain circuits and behavior. However, tools for highly controllable molecular release are lacking. Here, we developed a photoswitchable nanovesicle with azobenzene-containing phosphatidylcholine (azo-PC), coined ‘azosome’, for neuromodulation. Irradiation with 365 nm light triggers the trans-to-cis isomerization of azo-PC, resulting in a disordered lipid bilayer with decreased thickness and cargo release. Irradiation with 455 nm light induces reverse isomerization and switches the release off. Real-time fluorescence imaging shows controllable and repeatable cargo release within seconds (< 3 s). Importantly, we demonstrate that SKF-81297, a dopamine D1-receptor agonist, can be released from the azosome to activate cultures of primary striatal neurons. Azosome shows promise in precise optical control over the molecular release and can be a valuable tool for molecular neuroscience studies.

Competing Interest Statement

The authors have declared no competing interest.

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Posted June 12, 2022.
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Optical Control of Neuronal Activities with Photoswitchable Nanovesicles
Hejian Xiong, Kevin A. Alberto, Jonghae Youn, Jaume Taura, Johannes Morstein, Xiuying Li, Yang Wang, Dirk Trauner, Paul A. Slesinger, Steven O. Nielsen, Zhenpeng Qin
bioRxiv 2022.06.10.495373; doi: https://doi.org/10.1101/2022.06.10.495373
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Optical Control of Neuronal Activities with Photoswitchable Nanovesicles
Hejian Xiong, Kevin A. Alberto, Jonghae Youn, Jaume Taura, Johannes Morstein, Xiuying Li, Yang Wang, Dirk Trauner, Paul A. Slesinger, Steven O. Nielsen, Zhenpeng Qin
bioRxiv 2022.06.10.495373; doi: https://doi.org/10.1101/2022.06.10.495373

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