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PICLN modulates alternative splicing and ensures adaptation to light and temperature changes in plants

View ORCID ProfileJulieta L. Mateos, Sabrina E Sanchez, Martina Legris, View ORCID ProfileDavid Esteve-Bruna, Jeanette C. Torchio, View ORCID ProfileEzequiel Petrillo, View ORCID ProfileDaniela Goretti, View ORCID ProfileNoel Blanco-Touriñán, Danelle K. Seymour, View ORCID ProfileMarkus Schmid, Detlef Weigel, View ORCID ProfileDavid Alabadí, View ORCID ProfileMarcelo J. Yanovsky
doi: https://doi.org/10.1101/2022.06.14.496170
Julieta L. Mateos
1Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires–Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), C1405BWE Buenos Aires, Argentina
2Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET) and Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina
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  • For correspondence: jmateos@fbmc.fcen.uba.ar mjyanovsky@leloir.org.ar
Sabrina E Sanchez
1Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires–Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), C1405BWE Buenos Aires, Argentina
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Martina Legris
1Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires–Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), C1405BWE Buenos Aires, Argentina
6Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, CH-1015 Lausanne, Switzerland
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David Esteve-Bruna
3Instituto de Biología Molecular y Celular de Plantas (CSIC-Universidad Politecnica de Valencia), 46022 Valencia, Spain
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Jeanette C. Torchio
1Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires–Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), C1405BWE Buenos Aires, Argentina
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Ezequiel Petrillo
3Instituto de Biología Molecular y Celular de Plantas (CSIC-Universidad Politecnica de Valencia), 46022 Valencia, Spain
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Daniela Goretti
4Department of Plant Physiology, Umea Plant Science Centre, Umea University, SE-901 87 Umea, Sweden
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Noel Blanco-Touriñán
3Instituto de Biología Molecular y Celular de Plantas (CSIC-Universidad Politecnica de Valencia), 46022 Valencia, Spain
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Danelle K. Seymour
5Department of Molecular Biology, Max Planck Institute for Biology Tübingen, 72076 Tübingen, Germany
7Department of Botany and Plant Sciences, University of California, Riverside, Riverside, CA
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Markus Schmid
4Department of Plant Physiology, Umea Plant Science Centre, Umea University, SE-901 87 Umea, Sweden
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Detlef Weigel
5Department of Molecular Biology, Max Planck Institute for Biology Tübingen, 72076 Tübingen, Germany
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David Alabadí
3Instituto de Biología Molecular y Celular de Plantas (CSIC-Universidad Politecnica de Valencia), 46022 Valencia, Spain
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Marcelo J. Yanovsky
1Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires–Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), C1405BWE Buenos Aires, Argentina
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  • For correspondence: jmateos@fbmc.fcen.uba.ar mjyanovsky@leloir.org.ar
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Abstract

Plants undergo transcriptome reprogramming to adapt to daily and seasonal fluctuations in light and temperature conditions. While most efforts have focused on the role of master transcription factors, the importance of splicing factors modulating these processes is now emerging. Efficient pre-mRNA splicing depends on proper spliceosome assembly, which in plants and animals requires the PRMT5-methylosome complex. PICLN is part of the PRMT5-methylosome complex in both humans and Arabidopsis thaliana, and we show here that the human PICLN ortholog rescues phenotypes of A. thaliana picln mutants. Altered photomorphogenic and photoperiodic responses in A. thaliana picln mutants are associated with changes in pre-mRNA splicing, which partially overlap with those in prmt5 mutants. Mammalian PICLN also acts in concert with the Survival Motor Neuron (SMN) complex component GEMIN2 to modulate the late steps of UsnRNP assembly, and many alternative splicing events regulated by PICLN but not PRMT5 are controlled by A. thaliana GEMIN2. As with GEMIN2 and SME1/PCP, low temperature, which increases PICLN expression, aggravates morphological and molecular defects of picln mutants. Taken together, these results establish a key role for PICLN in the regulation of pre-mRNA splicing and in mediating plant adaptation to daily and seasonal fluctuations in environmental conditions.

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Posted June 16, 2022.
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PICLN modulates alternative splicing and ensures adaptation to light and temperature changes in plants
Julieta L. Mateos, Sabrina E Sanchez, Martina Legris, David Esteve-Bruna, Jeanette C. Torchio, Ezequiel Petrillo, Daniela Goretti, Noel Blanco-Touriñán, Danelle K. Seymour, Markus Schmid, Detlef Weigel, David Alabadí, Marcelo J. Yanovsky
bioRxiv 2022.06.14.496170; doi: https://doi.org/10.1101/2022.06.14.496170
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PICLN modulates alternative splicing and ensures adaptation to light and temperature changes in plants
Julieta L. Mateos, Sabrina E Sanchez, Martina Legris, David Esteve-Bruna, Jeanette C. Torchio, Ezequiel Petrillo, Daniela Goretti, Noel Blanco-Touriñán, Danelle K. Seymour, Markus Schmid, Detlef Weigel, David Alabadí, Marcelo J. Yanovsky
bioRxiv 2022.06.14.496170; doi: https://doi.org/10.1101/2022.06.14.496170

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