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Tunable control of insect pheromone biosynthesis in Nicotiana benthamiana

View ORCID ProfileKalyani Kallam, View ORCID ProfileElena Moreno-Giménez, Ruben Mateos-Fernández, Connor Tansley, Silvia Gianoglio, View ORCID ProfileDiego Orzaez, View ORCID ProfileNicola J. Patron
doi: https://doi.org/10.1101/2022.06.15.496242
Kalyani Kallam
1Engineering Biology, Earlham Institute, Norwich Research Park, Norwich, Norfolk, NR4 7UZ, UK
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  • ORCID record for Kalyani Kallam
Elena Moreno-Giménez
2Institute for Plant Molecular and Cell Biology (IBMCP), UPV-CSIC, Valencia, Spain
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Ruben Mateos-Fernández
2Institute for Plant Molecular and Cell Biology (IBMCP), UPV-CSIC, Valencia, Spain
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Connor Tansley
1Engineering Biology, Earlham Institute, Norwich Research Park, Norwich, Norfolk, NR4 7UZ, UK
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Silvia Gianoglio
2Institute for Plant Molecular and Cell Biology (IBMCP), UPV-CSIC, Valencia, Spain
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Diego Orzaez
2Institute for Plant Molecular and Cell Biology (IBMCP), UPV-CSIC, Valencia, Spain
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Nicola J. Patron
1Engineering Biology, Earlham Institute, Norwich Research Park, Norwich, Norfolk, NR4 7UZ, UK
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  • For correspondence: nicola.patron@earlham.ac.uk
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Abstract

Synthetic regulatory elements that provide control over the timing and levels of gene expression are useful for maximizing yields from heterologous biosynthetic pathways. Previous work has demonstrated that plants and microbes can be engineered to produce insect sex pheromones, providing a route for low-cost production of these compounds, which are valued for species-specific control of agricultural pests. Strong constitutive expression of pathway genes can lead to toxicity and metabolic loads, preventing normal growth and development and thus limiting biomass and affecting overall yields. In this study we demonstrate the ability to inducibly control the accumulation of Lepidopteran sex pheromones in leaves of Nicotiana benthamiana. Further, we show how construct architecture influences expression and product yields in multigene constructs, applying this to control the relative expression of genes within the pathway, thereby tuning the accumulation of pheromone components. The approaches demonstrated here provide new insights into the heterologous reconstruction of metabolic pathways in plants.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted June 15, 2022.
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Tunable control of insect pheromone biosynthesis in Nicotiana benthamiana
Kalyani Kallam, Elena Moreno-Giménez, Ruben Mateos-Fernández, Connor Tansley, Silvia Gianoglio, Diego Orzaez, Nicola J. Patron
bioRxiv 2022.06.15.496242; doi: https://doi.org/10.1101/2022.06.15.496242
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Tunable control of insect pheromone biosynthesis in Nicotiana benthamiana
Kalyani Kallam, Elena Moreno-Giménez, Ruben Mateos-Fernández, Connor Tansley, Silvia Gianoglio, Diego Orzaez, Nicola J. Patron
bioRxiv 2022.06.15.496242; doi: https://doi.org/10.1101/2022.06.15.496242

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