Abstract
Because overuse of nitrogen fertilizer made climate change and agriculture unsustainable, uncovering of signaling pathway under nitrogen deficiency conditions is essential. Here we cloned TaPCGR1-3B (Phospholipid Coordinated Growth and environment Response 1), controlled by SNPs on alternative splicing and transcription factors binding, conferring nitrogen deficiency response. TaPCGR1-3B was localized in plasma membrane and endoplasmic reticulum of meristem cell. Nitrogen deprivation stimulated interaction of TaPCGR1-3B with G protein alpha subunit and phospholipase C 9, which was inhibited by phosphatidylcholine, to trigger Ca2+ signaling and inhibit tiller elongation. Knockdown of TaPCGR1 rescued the grain loss caused by nitrogen deficient conditions by modulation of phosphatidylcholine induced tillering gene expression through Ca2+ signaling disruption. Reducing TaPCGR1 activity thus warranted an environmentally sustainable way for future agriculture and food security.
Competing Interest Statement
The authors have declared no competing interest.
