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Clock and riboswitch control of THIC in tandem are essential for appropriate gauging of TDP levels under light/dark cycles in Arabidopsis

View ORCID ProfileZeenat Noordally, Lara Land, Celso Trichtinger, Ivan Dalvit, Mireille de Meyer, Kai Wang, View ORCID ProfileTeresa B. Fitzpatrick
doi: https://doi.org/10.1101/2022.06.20.496815
Zeenat Noordally
Vitamins & Environmental Stress Responses in Plants, Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland
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  • ORCID record for Zeenat Noordally
Lara Land
Vitamins & Environmental Stress Responses in Plants, Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland
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Celso Trichtinger
Vitamins & Environmental Stress Responses in Plants, Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland
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Ivan Dalvit
Vitamins & Environmental Stress Responses in Plants, Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland
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Mireille de Meyer
Vitamins & Environmental Stress Responses in Plants, Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland
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Kai Wang
Vitamins & Environmental Stress Responses in Plants, Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland
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Teresa B. Fitzpatrick
Vitamins & Environmental Stress Responses in Plants, Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland
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  • ORCID record for Teresa B. Fitzpatrick
  • For correspondence: theresa.fitzpatrick@unige.ch
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SUMMARY

Metabolic homeostasis is regulated by enzyme activities but the importance of regulating their corresponding coenzyme levels is unexplored. The organic coenzyme thiamine diphosphate (TDP) is supplied as needed and controlled by a riboswitch sensing mechanism in plants through the circadian regulated THIC gene. Riboswitch disruption leads to loss of time-of-day regulation of THIC expression, negatively impacting plant fitness. Pathway precursor balancing combined with enhancing the biosynthesis pathway demonstrate the importance of the riboswitch in gauging TDP levels and indicate that TDP impacts the clock in Arabidopsis. Altering the phase of THIC expression to be synchronous with TDP transporters disrupts the precision of the riboswitch suggesting that temporal separation of these processes is important. All defects are bypassed by growing plants under continuous light conditions highlighting the need to control levels of this coenzyme under diel cycles. Thus, consideration of coenzyme homeostasis within the well-studied domain of metabolic homeostasis is highlighted.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted June 20, 2022.
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Clock and riboswitch control of THIC in tandem are essential for appropriate gauging of TDP levels under light/dark cycles in Arabidopsis
Zeenat Noordally, Lara Land, Celso Trichtinger, Ivan Dalvit, Mireille de Meyer, Kai Wang, Teresa B. Fitzpatrick
bioRxiv 2022.06.20.496815; doi: https://doi.org/10.1101/2022.06.20.496815
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Clock and riboswitch control of THIC in tandem are essential for appropriate gauging of TDP levels under light/dark cycles in Arabidopsis
Zeenat Noordally, Lara Land, Celso Trichtinger, Ivan Dalvit, Mireille de Meyer, Kai Wang, Teresa B. Fitzpatrick
bioRxiv 2022.06.20.496815; doi: https://doi.org/10.1101/2022.06.20.496815

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