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Germline Transmission of a Circular Human Artificial Chromosome in the Mouse

Aleksandra Wudzinska, Leslie A. Mitchell, Ran Brosh, Nazario Bosco, Francesco Boccalatte, Ioannis Aifantis, Sang Y. Kim, Teresa Davoli, Jef D. Boeke
doi: https://doi.org/10.1101/2022.06.22.496420
Aleksandra Wudzinska
1Institute for Systems Genetics NYU Langone Health, New York, NY 10016, USA
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Leslie A. Mitchell
1Institute for Systems Genetics NYU Langone Health, New York, NY 10016, USA
4Department of Research and Development, Neochromosome Inc, Long Island City, NY 11101
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Ran Brosh
1Institute for Systems Genetics NYU Langone Health, New York, NY 10016, USA
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Nazario Bosco
1Institute for Systems Genetics NYU Langone Health, New York, NY 10016, USA
5Biology Division, Volastra Therapeutics, New York, NY 10027
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Francesco Boccalatte
3Department of Pathology, NYU Grossman School of Medicine, New York, NY 10016, USA
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Ioannis Aifantis
3Department of Pathology, NYU Grossman School of Medicine, New York, NY 10016, USA
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Sang Y. Kim
3Department of Pathology, NYU Grossman School of Medicine, New York, NY 10016, USA
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Teresa Davoli
1Institute for Systems Genetics NYU Langone Health, New York, NY 10016, USA
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Jef D. Boeke
1Institute for Systems Genetics NYU Langone Health, New York, NY 10016, USA
2Department of Biomedical Engineering, NYU Tandon School of Engineering, Brooklyn, NY, USA
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  • For correspondence: jef.boeke@nyulangone.org
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Abstract

Although the structure and function of the alphoid-tetO Human Artificial Chromosome (tetO-HAC) has been previously described in cell culture models and somatically in the mouse, in vivo persistence and stability throughout meiosis and across generations were not evaluated. Here we report germline transmission of a circular tetO-HAC across three mouse generations without observable health or reproductive deficiencies. Furthermore, we show that the tetO-HAC is maintained without selection as an episome and can be efficiently transmitted by both ova and sperm.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted June 22, 2022.
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Germline Transmission of a Circular Human Artificial Chromosome in the Mouse
Aleksandra Wudzinska, Leslie A. Mitchell, Ran Brosh, Nazario Bosco, Francesco Boccalatte, Ioannis Aifantis, Sang Y. Kim, Teresa Davoli, Jef D. Boeke
bioRxiv 2022.06.22.496420; doi: https://doi.org/10.1101/2022.06.22.496420
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Germline Transmission of a Circular Human Artificial Chromosome in the Mouse
Aleksandra Wudzinska, Leslie A. Mitchell, Ran Brosh, Nazario Bosco, Francesco Boccalatte, Ioannis Aifantis, Sang Y. Kim, Teresa Davoli, Jef D. Boeke
bioRxiv 2022.06.22.496420; doi: https://doi.org/10.1101/2022.06.22.496420

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