New Results
RevGel-seq: instrument-free single-cell RNA sequencing using a reversible hydrogel for cell-specific barcoding
Jun Komatsu, Alba Cico, Raya Poncin, Maël Le Bohec, Jörg Morf, Stanislav Lipin, Antoine Graindorge, Hélène Eckert, Azadeh Saffarian, Léa Cathaly, Frédéric Guérin, Sara Majello, Damien Ulveling, Anaïs Vayaboury, Nicolas Fernandez, Dilyana Dimitrova, Xavier Bussell, Yannick Fourne, Pierre Chaumat, Barbara André, Elodie Baldivia, Ulysse Godet, Mathieu Guinin, Vivien Moretto, Joy Ismail, Olivier Caille, Natacha Roblot, Carine Beaupère, Alexandrine Liboz, Ghislaine Guillemain, Bertrand Blondeau, Pierre Walrafen, View ORCID ProfileStuart Edelstein
doi: https://doi.org/10.1101/2022.07.01.498266
Jun Komatsu
1Scipio bioscience, Paris, France
Alba Cico
1Scipio bioscience, Paris, France
Raya Poncin
1Scipio bioscience, Paris, France
Maël Le Bohec
1Scipio bioscience, Paris, France
Jörg Morf
1Scipio bioscience, Paris, France
3Skyhawk Therapeutics, Basel, Switzerland
Stanislav Lipin
1Scipio bioscience, Paris, France
Antoine Graindorge
1Scipio bioscience, Paris, France
Hélène Eckert
1Scipio bioscience, Paris, France
Azadeh Saffarian
1Scipio bioscience, Paris, France
Léa Cathaly
1Scipio bioscience, Paris, France
Frédéric Guérin
1Scipio bioscience, Paris, France
Sara Majello
1Scipio bioscience, Paris, France
Damien Ulveling
1Scipio bioscience, Paris, France
Anaïs Vayaboury
1Scipio bioscience, Paris, France
Nicolas Fernandez
1Scipio bioscience, Paris, France
Dilyana Dimitrova
1Scipio bioscience, Paris, France
Xavier Bussell
1Scipio bioscience, Paris, France
Yannick Fourne
1Scipio bioscience, Paris, France
Pierre Chaumat
1Scipio bioscience, Paris, France
Barbara André
1Scipio bioscience, Paris, France
Elodie Baldivia
1Scipio bioscience, Paris, France
Ulysse Godet
1Scipio bioscience, Paris, France
Mathieu Guinin
1Scipio bioscience, Paris, France
Vivien Moretto
1Scipio bioscience, Paris, France
Joy Ismail
1Scipio bioscience, Paris, France
Olivier Caille
1Scipio bioscience, Paris, France
Natacha Roblot
2Sorbonne Université, Inserm, Centre de Recherche Saint-Antoine, CRSA, F-75012 Paris, France
Carine Beaupère
2Sorbonne Université, Inserm, Centre de Recherche Saint-Antoine, CRSA, F-75012 Paris, France
Alexandrine Liboz
2Sorbonne Université, Inserm, Centre de Recherche Saint-Antoine, CRSA, F-75012 Paris, France
Ghislaine Guillemain
2Sorbonne Université, Inserm, Centre de Recherche Saint-Antoine, CRSA, F-75012 Paris, France
Bertrand Blondeau
2Sorbonne Université, Inserm, Centre de Recherche Saint-Antoine, CRSA, F-75012 Paris, France
Pierre Walrafen
1Scipio bioscience, Paris, France
Stuart Edelstein
1Scipio bioscience, Paris, France

ABSTRACT
Progress in sample preparation for scRNA-seq is reported based on RevGel-seq, a reversible-hydrogel technology. Barcode bead—cell tandems stabilized by a chemical linker are dispersed in the hydrogel in the liquid state. Upon gelation the tandems are immobilized, cell lysis is triggered by detergent diffusion, and RNA molecules are captured on the adjacent barcode beads. After reverse transcription and preparation for cDNA sequencing, bioinformatic analysis reveals performance quality comparable to microfluidic-based technologies.
Competing Interest Statement
Authors are employed by Scipio bioscience founded by corresponding authors Pierre Walrafen and Stuart Edelstein, except authors from St. Antoine Hospital who have no competing interests.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Posted July 02, 2022.
RevGel-seq: instrument-free single-cell RNA sequencing using a reversible hydrogel for cell-specific barcoding
Jun Komatsu, Alba Cico, Raya Poncin, Maël Le Bohec, Jörg Morf, Stanislav Lipin, Antoine Graindorge, Hélène Eckert, Azadeh Saffarian, Léa Cathaly, Frédéric Guérin, Sara Majello, Damien Ulveling, Anaïs Vayaboury, Nicolas Fernandez, Dilyana Dimitrova, Xavier Bussell, Yannick Fourne, Pierre Chaumat, Barbara André, Elodie Baldivia, Ulysse Godet, Mathieu Guinin, Vivien Moretto, Joy Ismail, Olivier Caille, Natacha Roblot, Carine Beaupère, Alexandrine Liboz, Ghislaine Guillemain, Bertrand Blondeau, Pierre Walrafen, Stuart Edelstein
bioRxiv 2022.07.01.498266; doi: https://doi.org/10.1101/2022.07.01.498266
RevGel-seq: instrument-free single-cell RNA sequencing using a reversible hydrogel for cell-specific barcoding
Jun Komatsu, Alba Cico, Raya Poncin, Maël Le Bohec, Jörg Morf, Stanislav Lipin, Antoine Graindorge, Hélène Eckert, Azadeh Saffarian, Léa Cathaly, Frédéric Guérin, Sara Majello, Damien Ulveling, Anaïs Vayaboury, Nicolas Fernandez, Dilyana Dimitrova, Xavier Bussell, Yannick Fourne, Pierre Chaumat, Barbara André, Elodie Baldivia, Ulysse Godet, Mathieu Guinin, Vivien Moretto, Joy Ismail, Olivier Caille, Natacha Roblot, Carine Beaupère, Alexandrine Liboz, Ghislaine Guillemain, Bertrand Blondeau, Pierre Walrafen, Stuart Edelstein
bioRxiv 2022.07.01.498266; doi: https://doi.org/10.1101/2022.07.01.498266
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