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Characterization of DNA replicators of a 75-kb region of Chromosome 2 of fission yeast, Schizosacharomyces pombe

View ORCID ProfileMukesh Pratap Yadav, Dharani Dhar Dubey
doi: https://doi.org/10.1101/2022.07.01.498468
Mukesh Pratap Yadav
*Department of Biotechnology, VBS Purvanchal University Jaunpur-India-222 003
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  • For correspondence: mukeshpyadava@gmail.com
Dharani Dhar Dubey
*Department of Biotechnology, VBS Purvanchal University Jaunpur-India-222 003
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Abstract

DNA replication origins of a 75-kb region of chromosome 2 of Schizosacharomyces pombe, are well characterized. Here, we have further characterized two origins, ars2-3358 and ars2-3363 on plasmids using an Autonomous Replicating Sequences (ARS) assay. Sequence analysis of the five ARSs located within the 75-kb region revealed several asymmetric AT motifs ≥10 bp in each of these origins. Interestingly, we have also found a 29 bp AT-rich sequence (29W) in ars2-3358, which alone is not sufficient to provide DNA replication origins to plasmid. A genomic search for asymmetric AT motifs ≥10 bp gave a total 2309 near matches to 9 of these motifs in the genome. Majority (>65% to >77%) of six of these AT motifs overlapped with the earlier mapped genome-wide DNA replication origins. Moreover, functional comparison of these AT motifs to the deletion and mutational studies showed strong correlation with DNA replication origins efficiency. Therefore, we hypothesize that these sequences are important redundant core elements of S. pombe origins.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • ↵Professor DD Dubey Retired from the Department of Biotechnology

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted July 03, 2022.
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Characterization of DNA replicators of a 75-kb region of Chromosome 2 of fission yeast, Schizosacharomyces pombe
Mukesh Pratap Yadav, Dharani Dhar Dubey
bioRxiv 2022.07.01.498468; doi: https://doi.org/10.1101/2022.07.01.498468
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Characterization of DNA replicators of a 75-kb region of Chromosome 2 of fission yeast, Schizosacharomyces pombe
Mukesh Pratap Yadav, Dharani Dhar Dubey
bioRxiv 2022.07.01.498468; doi: https://doi.org/10.1101/2022.07.01.498468

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